TY - JOUR AB - The Mediator complex, a multi‑subunit protein complex, plays an integral role in regulating transcription. Genetic alterations of the mediator subunit 15 (MED15) in separate tumor entities have been described previously. However, till now, not much is known about the role of MED15 in urothelial bladder cancer (BCa). Using cBioPortal, database analysis was executed for the mRNA expression and survival analysis of MED15 in BCa. Immunohistochemistry (IHC) analysis against MED15 was performed on tissue microarrays with 18 benign, 126 BCa, and 38 metastases samples. The intensity evaluation was performed using a staining intensity score from 0 to 3 and associated with clinicopathological data. The BCa cell lines T24 and TCCSUP were used for the functional investigation. After the MED15 knockdown by small interfering (si)RNA, cell proliferation, migration and invasion were investigated. On the mRNA level, only a low number of alterations (2%) was found for MED15 in BCa. Due to the small count of events, there were no significant differences or tendencies in survival. For IHC, MED15 was found to have a higher expression in non‑muscle invasive BCa compared with benign and muscle invasive BCa. For survival analysis, no significant differences between samples with or without overexpression of MED15 were found. In the functional analysis, proliferation, migration, and invasion were significantly reduced in BCa‑cells following the transient siRNA‑mediated MED15 knockdown. In summary, MED15 appears to play a role in the tumor parameters proliferation, migration, and invasion in BCa, but further investigations are necessary. AD - Clinic for Urology and Paediatric Urology, University Hospital of Bonn, D‑53127 Bonn, Germany Institute of Pathology, University Hospital of Bonn, D‑53127 Bonn, Germany AU - Syring,Isabella AU - Weiten,Richard AU - Müller,Tim AU - Schmidt,Doris AU - Steiner,Susanne AU - Kristiansen,Glen AU - Müller,Stefan,C. AU - Ellinger,Jörg DA - 2018/09/01 DO - 10.3892/ol.2018.9014 EP - 3021 IS - 3 JO - Oncol Lett KW - mediator complex subunit MED15 urothelial bladder cancer PY - 2018 SN - 1792-1074 1792-1082 SP - 3013 ST - The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy T2 - Oncology Letters TI - The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy UR - https://doi.org/10.3892/ol.2018.9014 VL - 16 ER -