TY - JOUR AB - Endometrioid endometrial carcinoma, commonly known as type 1 endometrial cancer, accounts for >80% of endometrial carcinomas and is dependent on estrogen. We recently reported on the prognostic significance of the BIRC5 survivin gene in endometrial cancer. Estradiol induces survivin expression in estrogen receptor‑positive, but not in estrogen receptor‑negative, cancer cells. Kaempferol, a bioflavonoid, reportedly inhibits estrogen receptor‑α (ERα) in hormone receptor‑positive breast cancer cells. However, whether kaempferol‑mediated inhibition of ERα suppresses survivin and induces cell death in endometrial cancer remains unclarified. The present study evaluated the antitumor effects of kaempferol on endometrial cancer cells. Cell viability assays, flow cytometry analysis, western blotting and annexin V analyses were used to analyze the antitumor effects of kaempferol. The results demonstrated that kaempferol successfully suppressed the viability of two ER‑positive endometrial cancer cell lines, with IC50 values of 83 and 65 µM. In addition, kaempferol induced sub‑G1 cell accumulation and apoptotic cell death (P<0.01) in a dose‑dependent manner. Treatment of cells with estradiol significantly induced co‑expression of nuclear ERα and survivin proteins (P<0.001). Further evaluation revealed that kaempferol causes apoptotic cell death largely by suppressing ERα, survivin and Bcl‑2 protein. Therefore, the results of the present study suggested that targeting ERα and survivin with kaempferol may be a novel therapeutic option against endometrial carcinoma. AD - Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo 113‑8655, Japan Kanagawa Health Service Association, Kanagawa 231‑0021, Japan Department of Obstetrics and Gynecology, Nihon University, Tokyo 173‑8610, Japan AU - Chuwa,Agapiti,Hipoliti AU - Sone,Kenbun AU - Oda,Katsutoshi AU - Tanikawa,Michihiro AU - Kukita,Asako AU - Kojima,Machiko AU - Oki,Shinya AU - Fukuda,Tomohiko AU - Takeuchi,Makoto AU - Miyasaka,Aki AU - Kashiyama,Tomoko AU - Ikeda,Yuji AU - Nagasaka,Kazunori AU - Mori‑Uchino,Mayuyo AU - Matsumoto,Yoko AU - Wada‑Hiraike,Osamu AU - Kuramoto,Hiroyuki AU - Kawana,Kei AU - Osuga,Yutaka AU - Fujii,Tomoyuki DA - 2018/11/01 DO - 10.3892/ol.2018.9340 EP - 6201 IS - 5 JO - Oncol Lett KW - kaempferol 17β‑estradiol estrogen receptor survivin endometrial cancer PY - 2018 SN - 1792-1074 1792-1082 SP - 6195 ST - Kaempferol, a natural dietary flavonoid, suppresses 17β‑estradiol‑induced survivin expression and causes apoptotic cell death in endometrial cancer T2 - Oncology Letters TI - Kaempferol, a natural dietary flavonoid, suppresses 17β‑estradiol‑induced survivin expression and causes apoptotic cell death in endometrial cancer UR - https://doi.org/10.3892/ol.2018.9340 VL - 16 ER -