TY - JOUR AB - MicroRNAs have been suggested as potential regulators in gastric cancer (GC) development through affecting the expression of their target genes. Previous studies have demonstrated that miR‑140‑5p is downregulated in GC. However, the underlying functional role of miR‑140‑5p in GC remains largely unknown. The present study revealed that miR‑140‑5p expression was significantly decreased in 60 GC tissues, compared with corresponding adjacent non‑tumor tissues. A lower miR‑140‑5p expression was significantly associated with lymph node metastasis and an advanced Tumor‑Node‑Metastasis stage in patients with GC. Furthermore, patients with a lower miR‑140‑5p expression exhibited shorter disease‑free survival and overall survival times. Gain‑ and loss‑of‑function assays revealed that increased miR‑140‑5p expression significantly inhibited GC cell proliferation and invasion ability, as well as the Wnt/β‑catenin signaling pathway by decreasing WNT1 and β‑catenin expression. However, decreasing miR‑140‑5p expression had the opposite effects. Bioinformatics methods and dual‑luciferase reporter assays revealed that WNT1 was a direct target of miR‑140‑5p. miR‑140‑5p suppressed cell proliferation and invasion by regulating WNT1 expression. Therefore, the results of the present study demonstrated that miR‑140‑5p may serve as a potential prognostic marker and therapeutic target in patients with GC. AD - Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, Huizhou, Guangdong 516000, P.R. China AU - Cha,Yinlian AU - He,Ying AU - Ouyang,Kaobin AU - Xiong,Hailin AU - Li,Jun AU - Yuan,Xia DA - 2018/11/01 DO - 10.3892/ol.2018.9480 EP - 6376 IS - 5 JO - Oncol Lett KW - gastric cancer microRNA‑140‑5p WNT1 cell proliferation cell invasion PY - 2018 SN - 1792-1074 1792-1082 SP - 6369 ST - MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway T2 - Oncology Letters TI - MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway UR - https://doi.org/10.3892/ol.2018.9480 VL - 16 ER -