TY - JOUR AB - MicroRNAs (miRNAs) are small non‑coding RNAs of 18‑25 nucleotides that modulate gene expression at the post‑transcriptional level. Grape seed proanthocyanidins (GSPs), which are biologically active components in grape seeds, have been demonstrated to exhibit anticancer effects. The current study investigated whether GSPs can regulate miRNA expression and the possible anticancer molecular mechanisms of GSPs. Pancreatic cancer (PC) cell samples, SS3, SS12 and SS24, were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control samples, SC3, SC12 and SC24, were also prepared. Using miRNA‑seq, transcriptome analysis identified 24, 83 and 83 differentially expressed (DE) miRNAs in SS3 vs. SC3, SS12 vs. SC12 and SS24 vs. SC24, respectively. This indicated that treatment with GSPs could modulate the expression of miRNAs. Subsequently, 74, 598 and 1,204 target genes for the three sets of DE miRNAs were predicted. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that multiple target genes were associated with the proliferation and apoptosis of PC cells. In addition, a network was constructed of the DE miRNAs and the target genes associated with PC. The associations identified suggested that treatment with GSPs may inhibit the proliferation of PC cells through the modulation of miRNA expression. AD - College of Life Science, Tarim University, Alar, Xinjiang 843300, P.R. China Center for Genome Analysis, ABLife Inc., Wuhan, Hubei 430075, P.R. China AU - Wang,Weihua AU - Zhan,Leilei AU - Guo,Dongqi AU - Xiang,Yanju AU - Tian,Muxing AU - Zhang,Yu AU - Wu,Hong AU - Wei,Yaxun AU - Ma,Ganglong AU - Han,Zhanjiang DA - 2019/03/01 DO - 10.3892/ol.2019.9887 EP - 2787 IS - 3 JO - Oncol Lett KW - microRNAs grape seed proanthocyanidins pancreatic cancer cells microRNA sequencing proliferation PY - 2019 SN - 1792-1074 1792-1082 SP - 2777 ST - Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression T2 - Oncology Letters TI - Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression UR - https://doi.org/10.3892/ol.2019.9887 VL - 17 ER -