TY - JOUR AB - Adamantinomatous craniopharyngioma (ACP) is a benign epithelial tumor of the sellar region. Whether primary human cell cultures can be used as a stable research model has yet to be determined. The characteristics of three cultured craniopharyngioma primary cell (CPC) lines were identified using immunofluorescence. The culture duration for each CPC line was 10, 20 and 30 days. Cell lines and paired parental tumor tissues were subsequently analyzed using transcriptome sequencing (RNA‑Seq). Transcriptomic differences between ACP tissues and CPC lines were compared. CPCs maintained the original epithelial lineage markers, including pan‑cytokeratin and epithelial cell adhesion molecule. However, the Pearson's correlation coefficient of transcriptomes between each pair of CPC lines and ACP tissues decreased from 0.657 (cultured for 10 days) to 0.61 (cultured for 20 days) and further to 0.547 (cultured for 30 days). The number of differentially expressed genes between ACP tissues and CPCs was increased from 1,247 (cultured for 10 days) to 1,643 (cultured for 20 days) and then to 1,949 (cultured for 30 days). The results of Gene Set Enrichment Analysis demonstrated that the diversity of gene sets increased with longer culture time. Significant differences in the majority of signature gene sets were not observed between ACP tissues and CPCs, with the exception of keratinization phenotype [normalized enrichment score (NES)=‑2.02, false discovery rate (FDR)=0.0038] and epithelial cell phenotype (NES=‑1.82, FDR=0.032). Cell proliferation (NES=1.78, FDR=0.028) and mitosis (NES=1.93, FDR=0.012) were enhanced in CPCs. Therefore, primary human cell cultures can be used as a suitable research platform for ACP, however further experiments are required. AD - Department of Neurosurgery, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China AU - Zhang,Pei‑Dong AU - Wang,Chao‑Hu AU - Fan,Jun AU - Peng,Jun‑Xiang AU - Pan,Jun AU - Qi,Song‑Tao AU - Liu,Yi DA - 2020/03/01 DO - 10.3892/ol.2020.11309 EP - 2354 IS - 3 JO - Oncol Lett KW - adamantinomatous craniopharyngioma primary cell cultures RNA‑Seq gene set enrichment analysis model PY - 2020 SN - 1792-1074 1792-1082 SP - 2346 ST - Feasibility of primary human cell cultures as a model for adamantinomatous craniopharyngioma research: Evidence from RNA‑Seq analysis T2 - Oncology Letters TI - Feasibility of primary human cell cultures as a model for adamantinomatous craniopharyngioma research: Evidence from RNA‑Seq analysis UR - https://doi.org/10.3892/ol.2020.11309 VL - 19 ER -