TY - JOUR
AB - Melanoma is a common type of cutaneous tumor, but current drug treatments do not satisfy clinical practice requirements. At present, mitochondrial uncoupling is an effective antitumor treatment. Triclosan, a common antimicrobial, also acts as a mitochondrial uncoupler. The aims of the present study were to investigate the effects of triclosan on melanoma cells and the underlying mechanisms. Mitochondrial membrane potential (MMP), mitochondrial morphology, mitochondrial reactive oxygen species (mito‑ROS), intracellular superoxide anion and [Ca2+]i were measured using confocal microscopy. It was found that triclosan application was associated with decreased A375 cell viability in a dose‑ and time‑dependent manner and these effects may have cell specificity. Furthermore, triclosan induced MMP depolarization, ATP content decrease, mito‑ROS and [Ca2+]i level increases, excessive mitochondrial fission, AMP‑activated protein kinase (AMPK) activation and STAT3 inhibition. Moreover, these aforementioned effects were reversed by acetylcysteine treatment. Triclosan acute treatment also induced mitochondrial swelling, which was reversed after AMPK‑knockdown associated with [Ca2+]i overload. Cell death was caused by STAT3 inhibition but not AMPK activation. Moreover, triclosan induced autophagy via the ROS/AMPK/p62/microtubule‑associated protein 1A/1B‑light chain 3 (LC3) signaling pathway, which may serve a role in feedback protection. Collectively, the present results suggested that triclosan increased mito‑ROS production in melanoma cells, following induced cell death via the STAT3/Bcl‑2 pathway and autophagy via the AMPK/p62/LC3 pathway.
AD - Department of Urology, The Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China
Department of Central Laboratory, The Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China
Department of Pathology, The Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China
Department of Clinical Laboratory, The Affiliated Hospital of Jiaxing University, Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China
Department of Pharmacology, College of Medical, Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China
AU - Jin,Jing
AU - Chen,Naiwen
AU - Pan,Huan
AU - Xie,Wenhua
AU - Xu,Hong
AU - Lei,Siyu
AU - Guo,Zhiqin
AU - Ding,Renye
AU - He,Yi
AU - Gao,Jinlai
DA - 2020/10/01
DO - 10.3892/ol.2020.11934
IS - 4
JO - Oncol Lett
KW - triclosan
apoptosis
autophagy
melanoma
PY - 2020
SN - 1792-1074
1792-1082
SP - 73
ST - Triclosan induces ROS‑dependent cell death and autophagy in A375 melanoma cells
T2 - Oncology Letters
TI - Triclosan induces ROS‑dependent cell death and autophagy in A375 melanoma cells
UR - https://doi.org/10.3892/ol.2020.11934
VL - 20
ER -