TY - JOUR AB - Oral squamous cell carcinoma is one of the most common causes of malignancy‑associated death. Early diagnosis of oral squamous cell carcinoma (OSCC) is important in patient treatment and prognostic evaluation. Due to the lack of significant therapeutic benefit, the 5‑year survival rate has not improved. Therefore, effective novel markers are needed to improve diagnosis. To determine novel promising diagnostic biomarkers for OSCC, 416 upregulated and 416 downregulated differentially expressed genes were screened from OSCC tissues using an RNA microarray. The results suggested that minichromosome maintenance protein (MCM5) mRNA was significantly overexpressed in OSCC tissues compared with that in adjacent normal tissues. Moreover, silencing of MCM5 expression an OSCC cell line (SCC‑15) significantly impaired proliferation and colony formation. Furthermore, negative regulation of the mRNA and protein expression of MCM5 and demonstrated that MCM5 served as a cancer‑promoting gene modulating OSCC cell proliferation through induced G2/M phase arrest. In this process, the mRNA expression of cyclin E and cyclin‑dependent kinase 2 was downregulated, while p21 expression was upregulated. These results suggested that MCM5 may be an important pathogenic factor of OSCC. High expression levels of MCM5 may serve as a marker for the early diagnosis of OSCC. AD - Scientific Research Center, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China Department of Stomatology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China AU - Hao,Miao AU - Wang,Huiyu AU - Zhang,Chu AU - Li,Chunyan AU - Wang,Xiaofeng AU - Hao,Miao AU - Wang,Huiyu AU - Zhang,Chu AU - Li,Chunyan AU - Wang,Xiaofeng DA - 2020/10/01 DO - 10.3892/ol.2020.11970 IS - 4 JO - Oncol Lett KW - minichromosome maintenance protein 5 cell cycle oral squamous cell carcinoma biomarker microarray PY - 2020 SN - 1792-1074 1792-1082 SP - 109 ST - Minichromosome maintenance protein 5 is an important pathogenic factor of oral squamous cell carcinoma T2 - Oncology Letters TI - Minichromosome maintenance protein 5 is an important pathogenic factor of oral squamous cell carcinoma UR - https://doi.org/10.3892/ol.2020.11970 VL - 20 ER -