TY - JOUR AB - Placenta-specific 8 (PLAC8) is closely associated with the proliferation, apoptosis and autophagy of several tumor cells. However, the expression and function of PLAC8 in oral squamous cell carcinoma (OSCC) remain unknown. Therefore, the present study investigated the function and mechanism of PLAC8 in OSCC. Reverse transcription-quantitative PCR and western blot analyses were performed to quantify the expression of PLAC8 in OSCC cell lines. The function of PLAC8 in OSCC was investigated via transfection, the Transwell and Cell Counting Kit-8 assays, immunofluorescence staining and western blotting. The results demonstrated that PLAC8 exspression was downregulated in OSCC cell lines. PLAC8 inhibited the cell proliferation in OSCC. In addition, PLAC8 restrained invasion and epithelial-mesenchymal transition of OSCC cells. Furthermore, β-catenin helped to repress PLAC8 expression by regulating the Wnt/β-catenin and PI3K/Akt/GSK3β signaling pathways in OSCC cells. Collectively, the results of the present study suggest that PLAC8 acts as a tumor suppressor in OSCC by downregulating β-catenin. AD - Department of Clinical Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China Department of Radiology, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China Department of Internal Medicine V Pulmonology, Allergology and Respiratory Critical Care Medicine, Saarland University, Homburg D-66421, Germany AU - Wu,Junlu AU - Wang,Xuetao AU - Shang,Anquan AU - Vella,Giovanna AU - Sun,Zujun AU - Ji,Ping AU - Yang,Dianyu AU - Wan,Aiming AU - Yao,Yiwen AU - Li,Dong DA - 2020/11/01 DO - 10.3892/ol.2020.11989 IS - 5 JO - Oncol Lett KW - placenta-specific 8 oral squamous cell carcinogenesis epithelial-mesenchymal transition Wnt/β-catenin PI3K/Akt/glycogen synthase kinase 3β PY - 2020 SN - 1792-1074 1792-1082 SP - 128 ST - PLAC8 inhibits oral squamous cell carcinogenesis and epithelial-mesenchymal transition via the Wnt/β-catenin and PI3K/Akt/GSK3β signaling pathways T2 - Oncology Letters TI - PLAC8 inhibits oral squamous cell carcinogenesis and epithelial-mesenchymal transition via the Wnt/β-catenin and PI3K/Akt/GSK3β signaling pathways UR - https://doi.org/10.3892/ol.2020.11989 VL - 20 ER -