TY - JOUR
AB - MicroRNAs (miRNAs) exert critical roles in the majority of biological and pathological processes. Recent studies have associated miR‑150 with a number of different cancer types. However, little is known about miR‑150 targets in cervical cancer. In the present study, the HeLa human cervical cancer cell line was transfected with hsa‑miR‑150‑5p mimics, hsa‑miR‑150‑5p inhibitors or miRNA controls. miR‑150 was predicted to bind the 3'untranslated region (3'UTR) of the CDKN1B gene, which encodes the cyclin‑dependent kinase inhibitor 1B (p27Kip1). The direct binding between miR‑150 and the 3'UTR of CDKN1B was confirmed using dual‑luciferase reporter assays. The effects of miR‑150 on CDKN1B mRNA expression, p27Kip1 protein expression, cell cycle and cell proliferation were determined using reverse‑transcription quantitative PCR, western blot analysis, flow cytometry and WST‑8 assays, respectively. miR‑150 was demonstrated to directly target the 3'UTR of CDKN1B in transfected HeLa cells. The expression of CDKN1B mRNA and p27Kip1 protein was reduced by miR‑150 mimics, and increased by miR‑150 inhibitors. Moreover, the overexpression of miR‑150 promoted cell cycle progression from the G0/G1 to the S phase and led to a significant increase in HeLa cell proliferation. The results of the present study indicated that miR‑150 promotes HeLa cell cycle progression and proliferation via the suppression of p27Kip1 expression.
AD - Department of Medical Technology and Sciences, International University of Health and Welfare, Narita Chiba 286‑8686, Japan
Department of Clinical Laboratory, Shikoku Central Hospital, Shikokuchuo, Ehime 799‑0193, Japan
Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa 761‑0123, Japan
AU - Oboshi,Wataru
AU - Hayashi,Keisuke
AU - Takeuchi,Hiroaki
AU - Ikeda,Katsuhide
AU - Yamaguchi,Yoshitaka
AU - Kimura,Asako
AU - Nakamura,Takehiro
AU - Yukimasa,Nobuyasu
DA - 2020/11/01
DO - 10.3892/ol.2020.12073
IS - 5
JO - Oncol Lett
KW - microRNA‑150
cervical cancer
HeLa cell
CDKN1B
p27Kip1
cell cycle
PY - 2020
SN - 1792-1074
1792-1082
SP - 210
ST - MicroRNA‑150 suppresses p27Kip1 expression and promotes cell proliferation in HeLa human cervical cancer cells
T2 - Oncology Letters
TI - MicroRNA‑150 suppresses p27Kip1 expression and promotes cell proliferation in HeLa human cervical cancer cells
UR - https://doi.org/10.3892/ol.2020.12073
VL - 20
ER -