TY - JOUR
AB - Nischarin is an integrin‑binding protein, which is well known as a novel tumor suppressor. In breast cancer, Nischarin serves a critical role in breast cancer cell migration and invasion. However, the molecular mechanism underlying the role of Nischarin remains unclear. Recent findings have demonstrated that epithelial‑mesenchymal transition (EMT) increases the capacity of cell migration and invasion. As a member of the integrin family, it was hypothesized that Nischarin may regulate cellular processes via various signaling pathways associated with the EMT process. The present study detected the mRNA levels of EMT regulators via reverse transcription‑quantitative PCR and related protein levels via western blotting in breast cancer cells, following NISCH‑overexpression and ‑knockdown. The results demonstrated that Nischarin inhibits cell proliferation, migration and invasion in breast cancer cells. Furthermore, when the NISCH gene was overexpressed, the relative mRNA level of E‑cadherin was increased, while the relative mRNA levels of several transcription factors, such as Snail, ZEB1, N‑cadherin, Slug, Twist1 and vimentin, decreased. When NISCH was silenced, these results were reversed. The present results demonstrated that Nischarin suppresses cell migration and invasion via inhibiting the EMT process.
AD - Department of Breast Surgery, Zhejiang Hospital, Hangzhou, Zhejiang 310030, P.R. China
Department of Breast Surgery, Hangzhou Cancer Hospital, Hangzhou, Zhejiang 310000, P.R. China
Department of Breast Surgery, Zhejiang University Affiliated Hangzhou First People Hospital, Hangzhou, Zhejiang 310000, P.R. China
AU - Cai,Yuan-Jie
AU - Ma,Bo
AU - Wang,Mei-Li
AU - Chen,Jie
AU - Zhao,Fu-Guang
AU - Zhou,Juan-Di
AU - Guo,Xu
AU - Zheng,Lei
AU - Xu,Chun-Jing
AU - Wang,Yi
AU - He,Yi-Bo
AU - Liu,Jian
AU - Xie,Shang-Nao
DA - 2020/12/01
DO - 10.3892/ol.2020.12154
IS - 6
JO - Oncol Lett
KW - breast cancer
Nischarin
epithelial‑mesenchymal transition
metastasis
migration
invasion
transcription factors
PY - 2020
SN - 1792-1074
1792-1082
SP - 291
ST - Impact of Nischarin on EMT regulators in breast cancer cell lines
T2 - Oncology Letters
TI - Impact of Nischarin on EMT regulators in breast cancer cell lines
UR - https://doi.org/10.3892/ol.2020.12154
VL - 20
ER -