TY - JOUR
AB - M2 macrophages serve roles in inhibiting inflammation and promoting tumor development. Reversing tumor‑associated macrophages (TAMs) from M2‑ to M1‑type polarization may provide an important strategy for tumor immunotherapy. The present study aimed to enhance antitumor immunity by targeting the concentration of iron in macrophages. Fe3O4‑based poly(lactic‑co‑glycolic) acid (PLGA) nanoparticles surface‑modified with an anti‑CD206 monoclonal antibody were prepared using the oil in water single‑emulsion technique. Particle size was measured using a particle size analyzer, the ζ potential was determined using a ζ potential analyzer and the carrier rate of Fe3O4 was measured using an iron assay kit. The conjugation of anti‑CD206, and the ability to target M2 macrophages were studied via immunofluorescence. Polarization indexes of the macrophages were detected using both western blotting and reverse transcription‑quantitative PCR (RT‑qPCR), and a mouse model with subcutaneous tumors was established to verify the antitumor effects of the nanoparticles in vivo. Nanoparticles had a mean diameter in the range of 260‑295 nm, and the ζ potential values were between ‑19 and ‑33 mV. The Fe3O4 association efficiency ranged from 65‑75%, whereas the anti‑CD206 conjunction efficiency ranged from 65‑70%. The immunofluorescence experiments were able to demonstrate the successful targeting of the M2 macrophages. The western blotting and RT‑qPCR experiments identified that CD206‑Fe3O4‑PLGA and Fe3O4‑PLGA promoted the expression of TNF‑α, inducible nitric oxide synthase (iNOS) and IL‑1β in the macrophages. The in vivo studies indicated that CD206‑Fe3O4‑PLGA nanoparticles were able to promote CD86 expression in TAMs, with CD86 being a specific marker of the M1 subtype. In summary, nanoparticles were characterized in the present study by their mean particle size, polydispersity index, ζ potential and morphology, as well as by their association with Fe3O4 and conjugation with the anti‑CD206 monoclonal antibody. Collectively, the present results suggested that the nanoparticles were able to both target M2 macrophages and reverse the M2 polarization of the macrophages to the M1 phenotype via the release of coated iron‑oxide particles.
AD - Department of Cardiothoracic Surgery and Abdominal Hernia Surgery, The People's Hospital of Kai Zhou District, Chongqing 400000, P.R. China
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400000, P.R. China
AU - Zhou,Yun
AU - Que,Ke-Ting
AU - Tang,Hua-Ming
AU - Zhang,Peng
AU - Fu,Qian-Mei
AU - Liu,Zuo-Jin
DA - 2020/12/01
DO - 10.3892/ol.2020.12161
IS - 6
JO - Oncol Lett
KW - iron oxide nanoparticle
poly(lactic‑co‑glycolic) acid
targeted drug delivery
tumor immunotherapy
PY - 2020
SN - 1792-1074
1792-1082
SP - 298
ST - Anti‑CD206 antibody‑conjugated Fe3O4‑based PLGA nanoparticles selectively promote tumor‑associated macrophages to polarize to the pro‑inflammatory subtype
T2 - Oncology Letters
TI - Anti‑CD206 antibody‑conjugated Fe3O4‑based PLGA nanoparticles selectively promote tumor‑associated macrophages to polarize to the pro‑inflammatory subtype
UR - https://doi.org/10.3892/ol.2020.12161
VL - 20
ER -