TY - JOUR AB - The function of microRNAs (miRs) is associated with the development and progression of various malignancies, with miRs presenting stably in the serum. The current study assessed the role of miR‑1246 and miR‑106b in the serum of patients with esophageal squamous cell carcinoma (ESCC). A comprehensive microarray analysis of miR expression was performed using the serum of patients with ESCC, which were subsequently validated via reverse transcription‑quantitative PCR. A total of 55 test samples were obtained from Chiba University and 101 validation samples were gained from Chiba Cancer Center. The results revealed that miR‑1246 expression significantly increased and miR‑106b expression significantly decreased in each cohort. Receiver operating characteristic analysis revealed that the area under the curve (AUC) value of miR‑1246 was 0.816 (sensitivity, 72.7%; specificity, 69.2%) and 0.779 (sensitivity, 71.3%; specificity, 70.6%) for the test and validation cohorts, respectively. The AUC of miR‑106b was 0.716 (sensitivity, 65.5%; specificity, 61.6%) and 0.815 (sensitivity, 74.3%; specificity, 73.5%), respectively. In addition, the AUC of the miR‑1246/miR‑106b ratio was 0.901 (sensitivity, 80.0%; specificity, 80.0%) and 0.903 (sensitivity, 82.1%; specificity, 82.3%), respectively, which indicated a higher diagnostic ability compared with that of miR‑1246 or miR‑106b alone. The high miR‑1246/miR‑106b ratio group was associated with clinicopathological factors such as depth of invasion, progression, lymph node metastasis, and poor prognosis. Therefore, effective biomarkers may be generated by combining individual miRs obtained by comprehensive analysis of ESCC patient sera. AD - Division of Gastroenterological Surgery, Chiba Cancer Center, Chuo‑ku, Chiba 260-8717, Japan Department of Hepatobiliary and Pancreatic Surgery, Chiba Cancer Center, Chuo‑ku, Chiba 260-8717, Japan Division of Surgical Technology, National Cancer Center Hospital East, Kashiwa, Chiba 277‑8577, Japan Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Chuo‑ku, Chiba 260‑8670, Japan AU - Hoshino,Isamu AU - Ishige,Fumitaka AU - Iwatate,Yosuke AU - Gunji,Hisashi AU - Shiratori,Fumiaki AU - Kuwayama,Naoki AU - Nabeya,Yoshihiro AU - Takeshita,Nobuyoshi AU - Matsubara,Hisahiro DA - 2020/12/01 DO - 10.3892/ol.2020.12213 IS - 6 JO - Oncol Lett KW - microRNA‑1246 microRNA‑106b esophageal squamous cell carcinoma serum PY - 2020 SN - 1792-1074 1792-1082 SP - 350 ST - Usefulness of serum miR‑1246/miR‑106b ratio in patients with esophageal squamous cell carcinoma T2 - Oncology Letters TI - Usefulness of serum miR‑1246/miR‑106b ratio in patients with esophageal squamous cell carcinoma UR - https://doi.org/10.3892/ol.2020.12213 VL - 20 ER -