TY - JOUR AB - Multiple myeloma (MM) is the second most common haematological malignancy and remains an incurable disease, with most patients relapsing and requiring further treatment. Augmenter of liver regeneration (ALR) is a vital protein affecting fundamental processes such as energy transduction, cell survival and regeneration. Silencing ALR inhibits cell proliferation and triggers apoptosis in human MM U266 cells. However, little is known about the role of 15‑kDa‑ALR on MM. In the present study, the role of 15‑kDa‑ALR in human MM cells was investigated. Blocking extracellular 15‑kDa‑ALR with an anti‑ALR monoclonal antibody (McAb) decreased the proliferation and viability of U266 cells. However, the results of flow cytometry revealed no changes in apoptosis, and the expression levels of Bax, Bcl‑2, caspase‑3 and cleaved caspase‑3 were not affected. However, combined treatment with anti‑ALR McAb and epirubicin increased the apoptosis of U266 cells. RNA sequencing results indicated that the ERK1/2, JNK‑MAPK and STAT3 signaling pathways, as well as the cell cycle, were associated with the mechanism of action of the anti‑ALR McAb, and PCR, western blotting and cell cycle analysis confirmed these results. The present findings suggested that blocking extracellular 15‑kDa‑ALR in U266 cells with an anti‑ALR McAb decreased cell proliferation via the MAPK, STAT3 and cell cycle signaling pathways without increasing apoptosis. Thus, 15‑kDa‑ALR may be a new therapeutic target for myeloma. AD - Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Department of Infectious Diseases, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China Department of Nephrology, The Affiliated Hospital of Panzhihua University, Panzhihua, Sichuan 617000, P.R. China AU - Huang,Wenqi AU - Sun,Hang AU - Hu,Ting AU - Zhu,Dongju AU - Long,Xianli AU - Guo,Hui AU - Liu,Qi DA - 2021/03/01 DO - 10.3892/ol.2021.12458 IS - 3 JO - Oncol Lett KW - ALR MM cells proliferation McAb PY - 2021 SN - 1792-1074 1792-1082 SP - 197 ST - Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway T2 - Oncology Letters TI - Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway UR - https://doi.org/10.3892/ol.2021.12458 VL - 21 ER -