TY - JOUR
AB - Chalcones and its derivatives are reported to exhibit anti‑cancer effects in several cancer cell lines, including colon cancer cells. However, the in vivo anticancer effects and associated mechanisms of chalcones against intestinal tumorigenesis currently remain unclear. The aim of the present study was to investigate the chemopreventive effect of a chalcone derivative, 4'‑hydroxychalcone (4‑HC), in a transgenic adenomatous polyposis coli multiple intestinal neoplasia mouse model (ApcMin) of spontaneous intestinal adenomas. ApcMin mice were fed 4‑HC (10 mg/kg/day) or the vehicle control by oral gavage starting at 8 weeks of age, and were sacrificed at 20 weeks. The administration of 4‑HC significantly decreased the number of colon adenomas by 45% and the size of colon adenomas by 35% compared with the respective controls. Similarly, the number of adenomas in the distal small intestine (DSI) and proximal small intestine also decreased by 35 and 33%, respectively, in 4‑HC‑treated mice, and adenoma size in the DSI decreased by 39% compared with the respective controls. Treatment with 4‑HC strongly decreased proliferation in colon and DSI adenomas, as detected by immunofluorescence staining with the proliferation marker protein Ki‑67, and promoted apoptosis in colon adenomas, as detected by TUNEL immunofluorescence staining. In addition, decreased mRNA expression of β‑catenin target genes, including c‑Myc, Axin2 and CD44, in colon adenomas of 4‑HC‑treated animals demonstrated the involvement of the Wnt/β‑catenin signaling pathway in the initiation and progression of colon neoplasms. Treatment with 4‑HC also decreased the protein levels of β‑catenin in colon adenomas, as demonstrated by immunofluorescence staining. The results suggested that 4‑HC may be a promising candidate for the chemoprevention of intestinal tumorigenesis, and further investigations are required to evaluate its clinical utility.
AD - Department of General Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Pediatrics, State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Department of General Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China
AU - Chen,Qing
AU - Lei,Jiahong
AU - Zhou,Jinzhe
AU - Ma,Shaoze
AU - Huang,Qi
AU - Ge,Bujun
DA - 2021/03/01
DO - 10.3892/ol.2021.12474
IS - 3
JO - Oncol Lett
KW - colorectal cancer
chalcone derivatives
chemoprevention
adenomatous polyposis coli
β‑catenin
PY - 2021
SN - 1792-1074
1792-1082
SP - 213
ST - Chemopreventive effect of 4'‑hydroxychalcone on intestinal tumorigenesis in ApcMin mice Corrigendum in /10.3892/ol.2021.12741
T2 - Oncology Letters
TI - Chemopreventive effect of 4'‑hydroxychalcone on intestinal tumorigenesis in ApcMin mice Corrigendum in /10.3892/ol.2021.12741
UR - https://doi.org/10.3892/ol.2021.12474
VL - 21
ER -