TY - JOUR AB - Triple‑negative breast cancer (TNBC) is a subtype with high rates of metastasis, poor prognosis and limited therapeutic options. The present study aimed to identify the potential pivotal genes for prognosis and treatment in TNBC. A total of two microarray expression datasets, GSE38959 and GSE65212, were downloaded from the Gene Expression Omnibus database, and RNA‑sequencing data of breast cancer from The Cancer Genome Atlas database were analyzed to screen out differentially expressed genes (DEGs) between TNBC tissues and normal tissues. The intersection of DEGs was submitted to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. A protein‑protein interaction (PPI) network was constructed and visualized using Cytoscape software. Furthermore, module, centrality and survival analyses were performed to identify the potential hub genes. Reverse transcription‑quantitative (RT‑q)PCR analysis was performed to detect the expression levels of key genes in TNBC samples, and 377 DEGs were identified. Functional analysis revealed that the DEGs were significantly involved in cell cycle process, nuclear division and the p53 signaling pathway. A PPI network was constructed with these DEGs, and 66 core genes with high centrality features in module 1 were selected. Relapse‑free survival analysis confirmed that high expression levels of five genes [cyclin B1 (CCNB1), GINS complex subunit 2, non‑SMC condensin I complex subunit G (NCAPG), minichromosome maintenance 4 (MCM4) and ribonucleotide reductase regulatory subunit M2 (RRM2)] were significantly associated with poor prognosis in TNBC. RT‑qPCR analysis demonstrated that CCNB1, NCAPG, MCM4 and RRM2 were significantly upregulated in 25 TNBC tissues compared with adjacent normal breast tissues. Furthermore, gene set enrichment analysis revealed that CCNB1, NCAPG, MCM4 and RRM2 were closely associated with tumor proliferation. Taken together, these results suggest that CCNB1, NCAPG, MCM4 and RRM2 are associated with tumorigenesis and TNBC progression, and thus may act as promising prognostic biomarkers and therapeutic targets for TNBC. AD - Department of Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, P.R. China Department of Bioinformatics, Chongqing Medical University, Chongqing 400016, P.R. China AU - Xiao,Xiao AU - Zhang,Zheng AU - Luo,Ruihan AU - Peng,Rui AU - Sun,Yan AU - Wang,Jia AU - Chen,Xin DA - 2021/05/01 DO - 10.3892/ol.2021.12624 IS - 5 JO - Oncol Lett KW - triple‑negative breast cancer bioinformatics analysis hub genes tumor proliferation prognosis PY - 2021 SN - 1792-1074 1792-1082 SP - 363 ST - Identification of potential oncogenes in triple‑negative breast cancer based on bioinformatics analyses T2 - Oncology Letters TI - Identification of potential oncogenes in triple‑negative breast cancer based on bioinformatics analyses UR - https://doi.org/10.3892/ol.2021.12624 VL - 21 ER -