TY - JOUR
AB - Aquaporin 1 (AQP1) contributes to the progression of several cancer types, but its potential involvement in triple‑negative breast cancer (TNBC) is unclear. The aim of the present study was to examine the role of AQP1 in cell proliferation and invasion in TNBC. Reverse transcription‑quantitative PCR analysis and western blotting were used to detect AQP1 expression in different cell lines. A short hairpin (sh)RNA targeting AQP1 was established and transfected into MDA‑MB‑231 breast cancer cells. To investigate the effects of AQP1 knockdown, breast cancer cell proliferation, migration and invasion were evaluated by Cell Counting Kit‑8 and Transwell assays. Furthermore, the volume and weight of tumor xenografts in mice were measured to evaluate breast cancer growth ability. The results revealed that the levels of AQP1 were higher in the MDA‑MB‑231 cell line compared with those in other breast cancer cell lines (MCF‑7 and SK‑BR‑3) and a normal mammary epithelial cell line (MCF‑10A). The shRNA targeting AQP1 effectively downregulated AQP1 expression at the mRNA and protein levels, and markedly suppressed TNBC cell proliferation, migration and invasion in vitro, and tumor growth in vivo. These results suggested that AQP1 may serve as a potential therapeutic target in TNBC.
AD - Department of Breast Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
AU - Ji,Yinan
AU - Liao,Xiaoming
AU - Jiang,Yi
AU - Wei,Wei
AU - Yang,Huawei
DA - 2021/06/01
DO - 10.3892/ol.2021.12698
IS - 6
JO - Oncol Lett
KW - triple‑negative breast cancer
AQP1
proliferation
migration
invasion
PY - 2021
SN - 1792-1074
1792-1082
SP - 437
ST - Aquaporin 1 knockdown inhibits triple‑negative breast cancer cell proliferation and invasion in vitro and in vivo
T2 - Oncology Letters
TI - Aquaporin 1 knockdown inhibits triple‑negative breast cancer cell proliferation and invasion in vitro and in vivo
UR - https://doi.org/10.3892/ol.2021.12698
VL - 21
ER -