TY - JOUR
AB - Pancreatic cancer is a highly malignant type of cancer and its treatment remains a major challenge. The novel recombinant protein TNF-related apoptosis-inducing ligand (TRAIL)-Mu3 has been shown to exert stronger tumor inhibitory effects in colon cancer in vitro and in vivo compared with TRAIL. The present study investigated the antitumor effects of TRAIL-Mu3 on pancreatic cancer cells, and the possible mechanisms were further examined. Compared with TRAIL, TRAIL-Mu3 exhibited significantly higher cytotoxic effects on pancreatic cancer cell lines. The inhibitory effect of TRAIL-Mu3 on the viability of PANC-1 cells was shown to be a caspase-dependent process. The affinity of TRAIL-Mu3 to PANC-1 cell membranes was significantly enhanced compared with TRAIL. In addition, TRAIL-Mu3 upregulated death receptor (DR) expression in PANC-1 cells and promoted the redistribution of DR5 in lipid rafts. Western blotting results demonstrated that TRAIL-Mu3 activated the caspase cascade in a faster and more efficient manner compared with TRAIL in PANC-1 cells. Therefore, TRAIL-Mu3 enhanced the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway. The present study indicated the potential of TRAIL-Mu3 for the treatment of pancreatic cancer.
AD - Department of Physiology, Chengdu Medical College, Chengdu, Sichuan 610000, P.R. China
Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu, Sichuan 610000, P.R. China
Chengdu Huachuang Biotechnology Co., Ltd., Chengdu, Sichuan 610000, P.R. China
Department of Neurology, The First People's Hospital of Longquanyi District, Chengdu, Sichuan 610000, P.R. China
Department of Pathophysiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610000, P.R. China
AU - Huang,Min
AU - Yi,Cheng
AU - Huang,Xian-Zhou
AU - Yan,Juan
AU - Wei,Li-Jia
AU - Tang,Wei-Ju
AU - Chen,Shou-Chun
AU - Huang,Ying
DA - 2021/06/01
DO - 10.3892/ol.2021.12699
IS - 6
JO - Oncol Lett
KW - tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)
TRAIL-Mu3
death receptor
apoptosis
pancreatic cancer
PY - 2021
SN - 1792-1074
1792-1082
SP - 438
ST - Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway
T2 - Oncology Letters
TI - Recombinant protein TRAIL-Mu3 enhances the antitumor effects in pancreatic cancer cells by strengthening the apoptotic signaling pathway
UR - https://doi.org/10.3892/ol.2021.12699
VL - 21
ER -