TY - JOUR
AB - Esophageal squamous cell carcinoma (ESCC) is a deadly squamous cell carcinoma (SCC) of the esophagus. Development of SCCs is associated with the deregulation of the squamous cell lineage program and/or keratinocyte terminal differentiation by genomic and genetic aberrations; thus, these processes must be tightly controlled to maintain normal squamous cell development. Zinc finger protein 750 (ZNF750) is a gene involved in keratinocyte terminal differentiation and is frequently mutated and putatively silenced in ESCC, which implicates its function as a potential differentiation‑related suppressor of ESCC. The present study aimed to elucidate the relationship between ZNF750 function to induce keratinocyte differentiation and tumor suppression in ESCC. The results demonstrated that chemical manipulation of esophageal keratinocyte differentiation in mouse normal esophageal epithelial organoids (mNEEO) implicated the involvement of the mouse homologue of ZNF750, Zfp750, in keratinocyte differentiation in premalignant cells. Bioinformatics analyses of data from high ZNF750‑expressing ESCC tumors obtained from public databases and ZNF750‑overexpressing ESCC cells compared with low ZNF750‑expressing ESCC tumors and GFP‑expressing ESCC cells, respectively, revealed enrichment of keratinocyte differentiation‑related gene sets in these samples. Finally, the induction through to terminal differentiation of the keratinocyte by all‑trans retinoic acid on parental ESCC cell lines led to the upregulation of the terminal differentiation marker Involucrin and a decrease in cell viability similar to that observed in ZNF750‑overexpressing ESCC cells. The results of the present study demonstrated a functional link between the ability of ZNF750 to induce cell differentiation through to terminal differentiation and its function as a growth suppressor in ESCC. This study provides improved understanding of the role of ZNF750, a frequently mutated differentiation‑related gene in ESCC, and its effects in ESCC pathogenesis.
AD - Department of Clinical Oncology, The University of Hong Kong, Hong Kong, SAR, P.R. China
AU - Ana Choi,Sheyne Sta
AU - Ko,Josephine,Mun‑Yee
AU - Yu,Valen,Zhuoyou
AU - Ning,Lvwen
AU - Lung,Maria,Li
DA - 2021/07/01
DO - 10.3892/ol.2021.12774
IS - 1
JO - Oncol Lett
KW - esophageal squamous cell carcinoma
zinc finger protein 750
keratinocyte differentiation
squamous cell carcinoma
tumor suppressor gene
PY - 2021
SN - 1792-1074
1792-1082
SP - 513
ST - Differentiation‑related zinc finger protein 750 suppresses cell growth in esophageal squamous cell carcinoma
T2 - Oncology Letters
TI - Differentiation‑related zinc finger protein 750 suppresses cell growth in esophageal squamous cell carcinoma
UR - https://doi.org/10.3892/ol.2021.12774
VL - 22
ER -