TY - JOUR
AB - N6‑methyladenosine (m6A), the most abundant internal RNA modification, serves a critical role in cancer development. However, the clinical implications of m6A in hepatocellular carcinoma (HCC) remain unclear. The present study sought to reveal the potential roles of m6A readers, which recognize m6A, in HCC. A total of 177 HCC and paired non‑cancerous liver tissues from patients who underwent hepatectomy were analysed using quantitative PCR for the expression of m6A readers: YT521‑B homology domain family 1 (YTHDF1) and YT521‑B homology domain family 2 (YTHDF2). The expression levels of both YTHDF1 and YTHDF2 were not significantly different between tumour and non‑cancerous tissues (P=0.93 and P=0.7, respectively). Analysis of the association between clinical features and m6A reader expression revealed that YTHDF1 expression was associated with formation of capsule (P=0.02), whereas low YTHDF2 expression was associated with septal formation (P=0.02). Furthermore, high YTHDF1 expression and high YTHDF2 expression were significantly associated with shorter recurrence‑free survival (RFS) [YTHDF1: Mean survival time (MST), 34.0 vs. 19.0 months, P=0.014; YTHDF2: MST, 30.1 vs. 12.9 months, P=0.0032], whereas YTHDF1 and YTHDF2 expression was not significantly associated with overall survival (OS) (YTHDF1: MST, 99.4 vs. 70.2 months, P=0.74; YTHDF2: MST, 98.4 vs. 64.1 months, P=0.28). According to multivariate analysis, serosal invasion [hazard ratio (HR), 2.39; 95% CI 1.30‑4.42; P=0.005), portal vein or hepatic vein invasion (HR, 2.82; 95% CI 1.26‑6.28; P=0.01) and YTHDF2 expression in HCC tissues (HR, 1.85; 95% CI 1.09‑3.15; P=0.02) were identified as significant independent prognostic factors for RFS. α‑fetoprotein (HR, 1.79; 95% CI 1.10‑2.92; P=0.02), serosal invasion (HR, 1.99; 95% CI 1.17‑3.34; P=0.01) and portal vein or hepatic vein invasion (HR, 3.02; 95% CI 1.38‑6.61; P=0.006) were identified as significant independent prognostic factors for OS. In conclusion, the present study revealed that high YTHDF2 expression, an m6A reader, in HCC tissues was associated with cancer recurrence.
AD - Department of Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466‑8550, Japan
AU - Nakagawa,Nobuhiko
AU - Sonohara,Fuminori
AU - Tanaka,Katsuhito
AU - Sunagawa,Yuki
AU - Inokawa,Yoshikuni
AU - Takami,Hideki
AU - Hayashi,Masamichi
AU - Yamada,Suguru
AU - Kanda,Mitsuro
AU - Tanaka,Chie
AU - Nakayama,Goro
AU - Koike,Masahiko
AU - Kodera,Yasuhiro
DA - 2021/07/01
DO - 10.3892/ol.2021.12799
IS - 1
JO - Oncol Lett
KW - hepatocellular carcinoma
methylation of N6 adenosine
YTH domain family
prognosis
PY - 2021
SN - 1792-1074
1792-1082
SP - 538
ST - Novel prognostic implications of YTH domain family 2 in resected hepatocellular carcinoma
T2 - Oncology Letters
TI - Novel prognostic implications of YTH domain family 2 in resected hepatocellular carcinoma
UR - https://doi.org/10.3892/ol.2021.12799
VL - 22
ER -