TY - JOUR AB - Colorectal cancer (CRC) is one of the most common malignancies worldwide. Via analysis using The Cancer Genome Atlas database, the present study identified 1,835 genes that were differentially expressed in CRC, including 811 upregulated and 1,024 downregulated genes. Enrichment analyses using the Database for Annotation, Visualization and Integrated Discovery tool revealed that these differentially expressed genes were associated with the regulation of CRC progression by modulating multiple pathways, such as ‘Cell Cycle, Mitotic’, ‘DNA Replication’, ‘Mitotic M‑M/G1 phases’ and ‘ATM pathway’. To identify the key genes in CRC, protein‑protein interaction (PPI) network analysis was performed and the hub modules in upregulated and downregulated PPI networks were identified. Ubiquitin‑conjugating enzyme E2 T (UBE2T), a member of the E2 family, was identified to be a key regulator in CRC. To the best of our knowledge, the present study was the first to demonstrate that UBE2T expression was upregulated in CRC samples compared with normal tissues. Kaplan‑Meier analysis revealed that higher expression levels of UBE2T were associated with worse prognosis compared with lower UBE2T expression levels in CRC. Additionally, the present study demonstrated that knockdown of UBE2T inhibited CRC cell proliferation. Flow cytometry assays revealed that UBE2T knockdown induced cell cycle arrest at G1 phase and apoptosis in vitro. These results suggested that UBE2T may be a novel potential biomarker for CRC. AD - Department of Gastroenterology, The Second Xiangya Hospital, Changsha, Hunan 410011, P.R. China AU - Luo,Min AU - Zhou,Yuqian DA - 2021/10/01 DO - 10.3892/ol.2021.12975 IS - 4 JO - Oncol Lett KW - colorectal cancer differentially expressed genes ubiquitin‑conjugating enzyme E2 T prognosis proliferation PY - 2021 SN - 1792-1074 1792-1082 SP - 714 ST - Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation T2 - Oncology Letters TI - Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation UR - https://doi.org/10.3892/ol.2021.12975 VL - 22 ER -