TY - JOUR
AB - Cholangiocarcinoma (CCA) is a highly aggressive and metastatic type of malignant carcinoma that is associated with high mortality rates and is difficult to detect at early stages. Core 3 structure is a mucin‑type O‑glycans synthesized by β1,3‑N‑acetylglucosaminyltransferase 6 (core 3 synthase), which plays an important role in the digestive system, in particular gastrointestinal goblet cells. It has been reported that core 3 synthase‑expressing cells show lower migratory and invasive rates, and lower metastatic activity. A immunohistochemical study also showed that this enzyme was expressed in normal epithelial cells of the colon, but completely disappeared in colorectal cancer cells. The present study aimed to identify biomarkers that could be used to predict the prognosis of patients with CCA. Pathological specimens of 185 CCA tissues were immunohistochemically stained with two antibodies, G8‑144 and MECA‑79, which recognize core 3 synthase and 6‑sulfated N‑acetyllactosamine on the extended core‑1 O‑glycans, respectively. The association between G8‑144 or MECA‑79 positivity and patient prognosis was statistically analyzed. Positive expression of G8‑144 was associated with improved prognosis in patients with distal CCA (dCCA). Patients with dCCA positive for G8‑144 showed lower mortality rates than those with negative expression. However, the positive expression of MECA‑79 was associated with CCA progression and metastasis, indicating that it is a poor prognostic marker for CCA. In conclusion, as both antibodies resulted in mirror‑image staining, the involvement of G8‑144 and MECA‑79 in O‑glycan synthesis could be considered as potential favorable and unfavorable biomarkers, respectively, for CCA prognosis.
AD - Graduate School of Comprehensive Human Sciences, Major in Medical Sciences, Clinical Sciences Program, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305‑8575, Japan
Division of Molecular Pathology, National Cancer Center Research Institute, Chuo‑ku, Tokyo 104‑0045, Japan
Hepatobiliary and Pancreatic Surgery Division, National Cancer Center Hospital, Chuo‑ku, Tokyo 104‑0045, Japan
Molecular and Cellular Glycoproteomics Research Group, Department of Life Science and Biotechnology, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305‑8565, Japan
AU - Boottanun,Patcharaporn
AU - Ino,Yoshinori
AU - Shimada,Kazuaki
AU - Hiraoka,Nobuyoshi
AU - Angata,Kiyohiko
AU - Narimatsu,Hisashi
DA - 2021/11/01
DO - 10.3892/ol.2021.13021
IS - 5
JO - Oncol Lett
KW - β1
3‑N‑acetylglucosaminyltransferase 6
core 3 synthase
immunohistochemistry
biomarker
cholangiocarcinoma
PY - 2021
SN - 1792-1074
1792-1082
SP - 760
ST - Association between the expression of core 3 synthase and survival outcomes of patients with cholangiocarcinoma
T2 - Oncology Letters
TI - Association between the expression of core 3 synthase and survival outcomes of patients with cholangiocarcinoma
UR - https://doi.org/10.3892/ol.2021.13021
VL - 22
ER -