TY - JOUR AB - Daidzein has been found to significantly inhibit the proliferation of lung cancer cells, while its potential molecular mechanisms remain unclear. To determine the molecular mechanism of daidzein on lung cancer cells, the Capital Bio Technology Human long non‑coding (lnc) RNA Array v4, 4x180K chip was used to detect the gene expression profiles of 40,000 lncRNAs and 34,000 mRNAs in a human cancer cell line. Reverse transcription‑quantitative (RT‑q) PCR analysis was performed to detect the expression levels of target lncRNA and mRNAs in the H1299 cells treated with and without daidzein, using the lncRNA and mRNA gene chip. Bioinformatics analysis was performed to determine the differentially expressed genes from the results of the chip assays. There were 119 and 40 differentially expressed lncRNAs and mRNAs, respectively, that had a 2‑fold change in expression level. A total of eight lncRNAs were upregulated in the H1299 lung cancer cells, while 111 lncRNAs were downregulated. Furthermore, five mRNAs were upregulated, and 35 mRNAs were downregulated. A total of six differentially expressed lncRNAs (ENST00000608897.1, ENST00000444196.1, ENST00000608741.1, XR_242163.1, ENST00000505196.1 and ENST00000498032.1) were randomly selected to validate the microarray data, which were consistent with the RT‑qPCR analysis results. Differentially expressed mRNAs were enriched in important Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways. Taken together, the results of the present study demonstrated that daidzein affected the expression level of lncRNAs in lung cancer cells, suggesting that daidzein may have potential effects on lung cancer cells. AD - Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang, Jiangxi 330006, P.R. China Department of Social Medicine and Public Health, School of Basic Medicine, Jiujiang University, Jiujiang, Jiangxi 332005, P.R. China AU - Li,Laifang AU - Liu,Jun AU - Wang,Xiaobo AU - Xiong,Xiaowei AU - Huang,Shaoxin AU - Wang,Xin DA - 2021/11/01 DO - 10.3892/ol.2021.13050 IS - 5 JO - Oncol Lett KW - daidzein long non‑coding RNA microarray analysis NSCLC differential expression PY - 2021 SN - 1792-1074 1792-1082 SP - 789 ST - Microarray analysis of differentially expressed long non‑coding RNAs in daidzein‑treated lung cancer cells T2 - Oncology Letters TI - Microarray analysis of differentially expressed long non‑coding RNAs in daidzein‑treated lung cancer cells UR - https://doi.org/10.3892/ol.2021.13050 VL - 22 ER -