TY - JOUR
AB - The Notch signaling pathway is crucial in both adipogenesis and tumor development. It serves a vital role in the development and stability of blood vessels and may be involved in the proliferative phase of infantile hemangiomas, which express various related receptors. Therefore, it was hypothesized that the Notch signaling pathway inhibitor N‑[N-(3,5‑difluorophenacetyl)‑L‑alanyl]‑S‑phenylglycine t‑butyl ester (DAPT), a γ‑secretase inhibitor, might help accelerate the regression of infantile hemangiomas. The present in vitro study evaluated whether inhibition of the Notch signaling pathway using DAPT could alter adipogenesis in hemangioma stem cells (HemSCs) derived from infantile hemangioma (IH) specimens. A total of 20 infants (age, ≤6 months) with hemangiomas who had not yet received any treatment were selected, and their discarded hemangioma tissues were obtained. HemSCs were isolated from the fresh, sterile IH specimens and treated with DAPT. Reverse transcription‑quantitative PCR and western blotting were used to demonstrate the inhibition of the Notch signaling pathway by DAPT. A proliferation assay (Cell Counting Kit‑8), oil red O staining, flow cytometry and a transwell assay were used to detect proliferation, adipogenesis, apoptosis and migration of HemSCs. Treatment with DAPT upregulated the expression levels of CCAAT/enhancer‑binding protein (C/EBP) α, C/EBPβ, peroxisome proliferator‑activated receptor‑γ, adiponectin and insulin‑like growth factor 1, and promoted the proliferation, apoptosis, migration and lipid accumulation in HemSCs in vitro. Targeting the Notch signaling pathway using DAPT may potentially accelerate the regression of infantile hemangiomas.
AD - Department of Plastic Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, P.R. China
Department of Anatomy, Anhui Medical University, Hefei, Anhui 230000, P.R. China
AU - Xu,Xing
AU - Wu,Yao
AU - Li,Honghong
AU - Xie,Juan
AU - Cao,Dongsheng
AU - Huang,Xueying
DA - 2021/12/01
DO - 10.3892/ol.2021.13115
IS - 6
JO - Oncol Lett
KW - Notch pathway
hemangioma
N-[N-(3
5‑difluorophenacetyl)-L‑alanyl]-S‑phenylglycine t‑butyl ester
γ‑secretase inhibitor
adipogenesis
regression
PY - 2021
SN - 1792-1074
1792-1082
SP - 854
ST - Notch pathway inhibitor DAPT accelerates in vitro proliferation and adipogenesis in infantile hemangioma stem cells
T2 - Oncology Letters
TI - Notch pathway inhibitor DAPT accelerates in vitro proliferation and adipogenesis in infantile hemangioma stem cells
UR - https://doi.org/10.3892/ol.2021.13115
VL - 22
ER -