TY - JOUR AB - Tumor‑associated macrophages (TAMs) are capable of worsening hepatocellular carcinoma (HCC) prognosis by accelerating tumor growth and progression. Signaling lymphocyte activation molecule family member 6 (SLAMF6; Ly108 in mice) is an immune regulator that is involved in numerous diseases. However, whether SLAMF6 might affect macrophage function in HCC has not yet been reported. Therefore, the present study aimed to determine the relationship between SLAMF6 expression on macrophages and HCC progression. In the present study, the expression of SLAMF6 in human blood samples and mice was analyzed by flow cytometry. Furthermore, macrophage‑related polarization markers were detected via reverse transcription quantitative PCR. Clonogenic formation and Transwell assay were performed to determine the proliferation, migration and invasion of HCC cells. In addition, a murine HCC model was established to detect the function of SLAMF6 in vivo. The results demonstrated that SLAMF6 expression was increased in CD14+ cells obtained from patients with HCC. It was also determined that this increase was associated with a positive hepatitis B virus DNA status and high levels of α‑fetoprotein. Polarized TAMs from THP‑1 cells, murine peritoneal macrophages and murine bone marrow‑derived macrophages all exhibited higher levels of SLAMF6 compared with M1 cells. Furthermore, an increased expression of Ly108 was detected in macrophages obtained from mice tumor tissues, indicating that the tumor microenvironment may promote Ly108 expression and macrophage M2 polarization. Ly108 small interfering RNA was applied to macrophages, which resulted in the suppression of M2 polarization. Ly108‑silenced macrophages attenuated HCC cell migration and invasion and prevented tumor growth by inhibiting the nuclear factor‑κB pathway. Altogether, the results from the present study suggested that SLAMF6/Ly108 was upregulated in TAMs, which may in turn accelerate the development of HCC. AD - School of Cheeloo Clinical Medicine, Shandong University, Jinan, Shandong 250102, P.R. China Department of Intervention Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China Head and Neck Radiation Oncology Ward II, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China Surgical Department, Jinan Jiyang District Hospital of Traditional Chinese Medicine, Jinan, Shandong 251499, P.R. China The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China AU - Meng,Qi AU - Duan,Xiuyun AU - Yang,Qingchao AU - Xue,Dewen AU - Liu,Zihao AU - Li,Yuanyuan AU - Jin,Qingyan AU - Guo,Fang AU - Jia,Shijie AU - Wang,Zhaofeng AU - Yan,Wenjiang AU - Chang,Xu AU - Sun,Peng DA - 2022/03/01 DO - 10.3892/ol.2022.13203 IS - 3 JO - Oncol Lett KW - hepatocellular carcinoma signaling lymphocyte activation molecule family member 6/Ly108 M2 macrophage PY - 2022 SN - 1792-1074 1792-1082 SP - 83 ST - SLAMF6/Ly108 promotes the development of hepatocellular carcinoma via facilitating macrophage M2 polarization T2 - Oncology Letters TI - SLAMF6/Ly108 promotes the development of hepatocellular carcinoma via facilitating macrophage M2 polarization UR - https://doi.org/10.3892/ol.2022.13203 VL - 23 ER -