TY - JOUR AB - The aim of the present study was to explore the role and biological function of bromodomain PHD finger transcription factor (BPTF) in T‑cell lymphoma. Reverse transcription‑quantitative PCR (RT‑qPCR), western blotting, immunohistochemistry and bioinformatics analysis were used to determine the expression levels of BPTF and Raf1 in T‑cell lymphoma tissues and matched adjacent normal tissues. RT‑qPCR and western blot analyses were used to examine the role of BPTF in the activation of MAPK signaling. The function of BPTF and Raf1 in T‑cell lymphoma was investigated through in vitro and in vivo assays (MTT assay, colony formation assay, flow cytometry, western blotting, tumor xenograft model and TUNEL assay) following silencing and overexpression experiments in Hut‑102 cells. The results demonstrated that BPTF and Raf1 were overexpressed in T‑cell lymphoma tissues compared with normal tissues, and high expression of BPTF or Raf1 was associated with advanced clinical stage. BPTF promoted the activation of the MAPK pathway and was coexpressed with Raf1 in T‑cell lymphoma tissues. Functional assays demonstrated that silencing of BPTF or Raf1 in Hut‑102 cells suppressed cell proliferation and induced apoptosis. Furthermore, the carcinogenic effect of BPTF was confirmed by xenograft experiments in nude mice. The present findings suggested that BPTF may function as a crucial oncogenic factor and may serve as a novel therapeutic target in T‑cell lymphoma. AD - Department of Pathology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China Department of Hematology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China Department of Pathology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China Department of Pathology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150000, P.R. China AU - Bai,Dongyu AU - Zhou,Yong AU - Shen,Fayan AU - Gao,Dehong AU - Suo,Wenhao AU - Zhang,Haiping AU - Li,Heng DA - 2022/07/01 DO - 10.3892/ol.2022.13344 IS - 1 JO - Oncol Lett KW - bromodomain PHD finger transcription factor Raf1 MAPK pathway T‑cell lymphoma PY - 2022 SN - 1792-1074 1792-1082 SP - 223 ST - BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma T2 - Oncology Letters TI - BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T‑cell lymphoma UR - https://doi.org/10.3892/ol.2022.13344 VL - 24 ER -