TY - JOUR AB - Cervical cancer (CC) is one of the most common gynecological malignancies that endangers women's health. A negative effect of glycolysis is that it contributes to abnormal tumor growth. MicroRNA (miR)‑99a expression has been found to be decreased in CC. The present study aimed to investigate the role of miR‑99a‑5p in glycolysis in CC. For this purpose, the association between miR‑99a and the prognosis of patients with CC from The Cancer Genome Atlas database was analyzed using Kaplan‑Meier analysis. miR‑99a‑5p expression and Ras‑related GTP binding D (RRAGD) expression were assessed using reverse transcription‑quantitative PCR and western blot analysis. Cell proliferation and apoptosis were examined using an MTT assay and flow cytometry, respectively. Glucose uptake, lactate concentration and extracellular acidification rate were measured using a glucose uptake colorimetric assay, a lactate colorimetric assay and a Seahorse XFe96 extracellular flux analyzer, respectively. The association between miR‑99a‑5p and RRAGD was predicted using TargetScan 7.1, and was confirmed using dual luciferase reporter assay. The results revealed that miR‑99a‑5p expression was decreased and that of RRAGD was increased in CC tissues and cell lines. RRAGD was negatively regulated by miR‑99a‑5p. The overexpression of miR‑99a‑5p induced apoptosis and inhibited glycolysis in CC cells by targeting RRAGD. On the whole, the present study revealed a novel mechanism through which miR‑99a‑5p regulates cell apoptosis and glycolysis in CC, thus providing a potential therapeutic target for CC. AD - Department of Gynecology, Wuhan Third Hospital (Tongren Hospital of Wuhan University), Wuhan, Hubei 430061, P.R. China Department of Vasculocardiology, Wuhan Wuchang Hospital, Wuhan, Hubei 430061, P.R. China Department of Obstetrics, Wuhan Third Hospital (Tongren Hospital of Wuhan University), Wuhan, Hubei 430061, P.R. China AU - Wang,Gang AU - Lu,Yu AU - Di,Shi AU - Xie,Maohua AU - Jing,Fang AU - Dai,Xiaoyan DA - 2022/07/01 DO - 10.3892/ol.2022.13349 IS - 1 JO - Oncol Lett KW - microRNA‑99a‑5p cervical cancer cell apoptosis glycolysis Ras‑related GTP binding D PY - 2022 SN - 1792-1074 1792-1082 SP - 228 ST - miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD T2 - Oncology Letters TI - miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD UR - https://doi.org/10.3892/ol.2022.13349 VL - 24 ER -