TY - JOUR
AB - MUC13, a transmembrane mucin glycoprotein, is overexpressed in colorectal cancer (CRC), however, its regulation and functions are not fully understood. It has been shown that MUC13 protects colonic epithelial cells from apoptosis. Therefore, studying MUC13 and MUC13‑regulated pathways may reveal promising therapeutic approaches for CRC treatment. Growing evidence suggests that microRNAs (miRs) are involved in the development and progression of CRC. In the present study, the MUC13‑miR‑4647 axis was addressed in association with survival of patients. miR‑4647 is predicted in silico to bind to the MUC13 gene and was analyzed by RT‑qPCR in 187 tumors and their adjacent non‑malignant mucosa of patients with CRC. The impact of previously mentioned genes on survival and migration abilities of cancer cells was validated in vitro. Significantly upregulated MUC13 (P=0.02) in was observed tumor tissues compared with non‑malignant adjacent mucosa, while miR‑4647 (P=0.05) showed an opposite trend. Higher expression levels of MUC13 (log‑rank P=0.05) were associated with worse patient's survival. The ectopic overexpression of studied miR resulted in decreased migratory abilities and worse survival of cells. Attenuated MUC13 expression levels confirmed the suppression of colony forming of CRC cells. In summary, the present data suggested the essential role of MUC13‑miR‑4647 in patients' survival, and this axis may serve as a novel therapeutic target. It is anticipated MUC13 may hold significant potential in the screening, diagnosis and treatment of CRC.
AD - Department of Surgery, Thomayer Hospital, 14200 Prague, Czech Republic
Department of Molecular Biology of Cancer, Institute of Experimental Medicine of The Czech Academy of Sciences, 14200 Prague, Czech Republic
Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, 12108 Prague, Czech Republic
Centre for Phenogenomics, Institute of Molecular Genetics, BIOCEV, 25250 Vestec, Czech Republic
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, 30166 Pilsen, Czech Republic
Department of Surgery, University Hospital Kralovske Vinohrady and Third Faculty of Medicine, Charles University, 10034 Prague, Czech Republic
Department of Oncology and Radiotherapy, University Hospital and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech Republic
AU - Sojka,Ladislav
AU - Opattova,Alena
AU - Bartu,Linda
AU - Horak,Josef
AU - Korenkova,Vlasta
AU - Novosadova,Vendula
AU - Krizkova,Vera
AU - Bruha,Jan
AU - Liska,Vaclav
AU - Schneiderova,Michaela
AU - Kubecek,Ondrej
AU - Vodickova,Ludmila
AU - Urbanova,Marketa
AU - Simsa,Jaromir
AU - Vodicka,Pavel
AU - Vymetalkova,Veronika
AU - Sojka,Ladislav
AU - Opattova,Alena
AU - Bartu,Linda
AU - Horak,Josef
AU - Korenkova,Vlasta
AU - Novosadova,Vendula
AU - Krizkova,Vera
AU - Bruha,Jan
AU - Liska,Vaclav
AU - Schneiderova,Michaela
AU - Kubecek,Ondrej
AU - Vodickova,Ludmila
AU - Urbanova,Marketa
AU - Simsa,Jaromir
AU - Vodicka,Pavel
AU - Vymetalkova,Veronika
DA - 2023/02/01
DO - 10.3892/ol.2022.13658
IS - 2
JO - Oncol Lett
KW - colorectal cancer risk and clinical outcomes
MUC13
microRNA
translation research
PY - 2023
SN - 1792-1074
1792-1082
SP - 72
ST - MUC13‑miRNA‑4647 axis in colorectal cancer: Prospects to identifications of risk factors and clinical outcomes
T2 - Oncology Letters
TI - MUC13‑miRNA‑4647 axis in colorectal cancer: Prospects to identifications of risk factors and clinical outcomes
UR - https://doi.org/10.3892/ol.2022.13658
VL - 25
ER -