TY - JOUR AB - Malignant osteosarcoma (OS) is a tumor of bone and soft tissue that metastasizes early and has a high mortality rate. Protein serine kinase H1 (PSKH1), an autophosphorylating human protein serine kinase, controls the trafficking of serine/arginine‑rich domain, with downstream effects on mRNA processing. It is also associated with tumor progression. However, how this protein contributes to OS progression and metastasis is unknown. The present study evaluated the potential effect of PSKH1 on proliferation of human OS cells. OS cell lines were used in Cell Counting Kit‑8, colony formation, wound‑healing and Transwell assays, to investigate cellular processes such as proliferation, migration and invasion and underlying molecular mechanisms. Expression of PSKH1 in OS tissue was significantly greater than in adjacent non‑malignant tissue. PSKH1 knockdown inhibited the proliferation, migration and invasion of OS cells. Conversely, PSKH1 overexpression promoted proliferation of OS cells. PSKH1 upregulated phosphorylated‑p38 in OS cells. Moreover, the p38 MAPK inhibitor SB203580 effectively blocked the tumor‑promoting action of PSKH1. Furthermore, PSKH1 knockdown inhibited tumor growth and metastasis in vivo. In conclusion, these findings suggested that PSKH1 promoted OS proliferation, migration and invasion. Thus, PSKH1 may serve an oncogenic role in the development of human OS. AD - Department of Orthopedics, Tongji Hospital, Tongji University, Shanghai 200065, P.R. China AU - Zhu,Xingfei AU - Jiang,Chao AU - Wang,Zhiyuang AU - Zhu,Xiaozhong AU - Yuan,Feng AU - Yang,Yi DA - 2023/04/01 DO - 10.3892/ol.2023.13730 IS - 4 JO - Oncol Lett KW - osteosarcoma protein serine kinase H1 cell proliferation cell invasion p38 therapeutic target PY - 2023 SN - 1792-1074 1792-1082 SP - 144 ST - PSKH1 affects proliferation and invasion of osteosarcoma cells via the p38/MAPK signaling pathway T2 - Oncology Letters TI - PSKH1 affects proliferation and invasion of osteosarcoma cells via the p38/MAPK signaling pathway UR - https://doi.org/10.3892/ol.2023.13730 VL - 25 ER -