TY - JOUR AB - The p14ARF and p16INK4a genes are localized to 9p21, where genetic alterations have been reported to be frequent in various human neoplasms. To elucidate their status in salivary gland tumorigenesis, we analyzed a series of 36 salivary gland carcinomas (SGCs) using methylation-specific PCR, differential PCR and immunohistochemistry. Homozygous deletion (3 cases) or methylation (7 cases) of p14ARF was detected in 10 (28%) SGCs, one and three showing co-deletion and co-methylation of both p14ARF and p16INK4a genes, respectively. A total of 5 (14%) SGCs demonstrated homozygous deletion (1 case) or methylation (4 cases) of p16INK4a, all but one being adenoid cystic carcinomas. Immunohistochemical study revealed loss of p14ARF and p16INK4a expression in 11 samples (31%), correlating with the gene status. These results indicate that inactivation of p14ARF and p16INK4a genes by either homozygous deletion or promoter hypermethylation may be important for the molecular pathogenesis of salivary malignant tumors, and provide clear evidence that epigenetic changes like methylation are related to salivary gland carcinogenesis. AD - Department of Pathology, Nara Medical University, Kashihara, Nara 634-8521, Japan null AU - Nishimine,Masayoshi AU - Nakamura,Mitsutoshi AU - Kishi,Munehiro AU - Okamoto,Masumi AU - Shimada,Keiji AU - Ishida,Eiwa AU - Kirita,Tadaaki AU - Konishi,Noboru DA - 2003/05/01 DO - 10.3892/or.10.3.555 EP - 560 IS - 3 JO - Oncol Rep PY - 2003 SN - 1021-335X 1791-2431 SP - 555 ST - Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas T2 - Oncology Reports TI - Alterations of p14ARF and p16INK4a genes in salivary gland carcinomas UR - https://doi.org/10.3892/or.10.3.555 VL - 10 ER -