TY - JOUR AB - We have investigated the effect of lupeol (lup-20(29)-ene-3β-ene-3-ol) isolated from mokumen (Gossampinus malabarica L. Merr) on DNA of human promyelotic HL-60 leukemia cells. Induction of apoptosis by lupeol was observed in human leukemia HL-60 cells. Morphological change showing apoptotic bodies was observed in the HL-60 cells treated with lupeol. The fragmentation of DNA by lupeol to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in the HL-60 cells. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 28.5, 42.0 and 70.9% after a 3-day treatment with 75, 100 and 150 µM lupeol, respectively. The data of the present study show that the suppression by lupeol of growth of the HL-60 cells results from the induction of apoptosis by this compound. AD - Faculty of Bioresources, Mie University, Tsu-city, Mie 514-0001, Japan null AU - Aratanechemuge,Yue AU - Hibasami,Hiroshige AU - Sanpin,Koh AU - Katsuzaki,Hirotaka AU - Imai,Kunio AU - Komiya,Takashi DA - 2004/02/01 DO - 10.3892/or.11.2.289 EP - 292 IS - 2 JO - Oncol Rep PY - 2004 SN - 1021-335X 1791-2431 SP - 289 ST - Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells T2 - Oncology Reports TI - Induction of apoptosis by lupeol isolated from mokumen (Gossampinus malabarica L. Merr) in human promyelotic leukemia HL-60 cells UR - https://doi.org/10.3892/or.11.2.289 VL - 11 ER -