TY - JOUR AB - Metastasis-associated protein 1 (MTA1) is highly upregulated in cancer cells with metastatic potential; however, the molecular mechanism by which MTA1 increases the metastatic potential of cancer cells is unknown. We characterized the functional consequences of MTA1 overexpression in cancer cells with an emphasis on its potential role as a deacetylator of hypoxia-inducible factor-1α (HIF-1α). MTA1 increased the expression of HIF-1α protein, but did not increase the expression of its mRNA. Glutathione S-transferase pull-down and coimmunoprecipitation assays demonstrated direct interaction of MTA1 with HIF-1α both in vitro and in vivo. Immunoprecipitation and acetylation assays also showed that MTA1 has deacetylation activity on HIF-1α in vivo. Moreover, MTA1 increased the transcriptional activity of HIF-1α and enhanced the expression of vascular endothelial growth factor, a target molecule of HIF-1α. Conditioned medium collected from MTA1 transfectants also increased angiogenesis in vitro and in vivo, probably through enhanced HIF-1α stabilization. These results indicate that MTA1 enhances angiogenesis by stabilization of the HIF-1α protein, which is closely related to the increased metastatic potential of cancer cells with high MTA1 expression. AD - Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea null AU - Moon,Hyo-Eun AU - Cheon,Hwanju AU - Chun,Kwang-Hoon AU - Lee,Seung,Ki AU - Kim,Yong-Sik AU - Jung,Bo-Kyung AU - Park,Jeong,Ae AU - Kim,Se-Hee AU - Jeong,Joo-Won AU - Lee,Myung-Shik DA - 2006/10/01 DO - 10.3892/or.16.4.929 EP - 935 IS - 4 JO - Oncol Rep PY - 2006 SN - 1021-335X 1791-2431 SP - 929 ST - Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1α T2 - Oncology Reports TI - Metastasis-associated protein 1 enhances angiogenesis by stabilization of HIF-1α UR - https://doi.org/10.3892/or.16.4.929 VL - 16 ER -