TY - JOUR AB - Prohibitin (PHB) is a ubiquitous protein with a number of different molecular functions. PHB is involved in tumorigenesis by exerting either a permissive or blocking action on tumor growth, depending on the cell context. In the present study, we investigated the effects of the histone deacetylase inhibitors (HDACis), trichostatin A (TSA) and sodium butyrate (NaB), on PHB expression in the thyroid tumor cell lines, TPC-1 and FRO. Both TSA and NaB increased PHB mRNA levels. Transfection experiments showed that the overexpression of HDAC1 or 2, but not 3, inhibited PHB promoter activity. The effects of TSA and NaB on the two major PHB mRNA splicing isoforms, were also investigated. Both TSA and NaB decreased the mRNA levels of the shorter isoform, but increased those of the longer isoform. Only the latter isoform contains a 3'UTR, which has been reported to exert a growth suppressive action. Thus, our data demonstrate that HDACis control both PHB transcription and alternative splicing. The effect of HDACis on PHB alternative splicing was not due to the modification of the expression of the ASF/SF2 splicing factor. AD - Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Udine, Italy null Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine, Piazzale Kolbe 1, 33100 Udine, Italy AU - Puppin,Cinzia AU - Passon,Nadia AU - Franzoni,Alessandra AU - Russo,Diego AU - Damante,Giuseppe DA - 2011/02/01 DO - 10.3892/or.2010.1075 EP - 397 IS - 2 JO - Oncol Rep KW - thyroid tumors prohibitin transcription alternative splicing PY - 2011 SN - 1021-335X 1791-2431 SP - 393 ST - Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells T2 - Oncology Reports TI - Histone deacetylase inhibitors control the transcription and alternative splicing of prohibitin in thyroid tumor cells UR - https://doi.org/10.3892/or.2010.1075 VL - 25 ER -