TY - JOUR AB - To elucidate the potential etiological role of RUNX3 in the development of salivary gland adenoid cystic carcinoma (ACC), we analyzed the methylation status of RUNX3 in a series of 114 ACC tissues and 3 ACC cell lines. Results showed that the methylated rate of RUNX3 was 50.9 and 3.5% in the 114 ACC samples and the corresponding normal salivary glands, respectively, achieving a significant difference (P<0.001). There was a significant correlation between RUNX3 methylation and various clinicopathological parameters of ACCs, such as perineural invasion, lymph node involvement, T-stage and distant metastasis (P<0.001). RUNX3 methylation was a significant predictor of the 5-year disease-free survival in ACC patients after surgery. Partial methylation was found in all 3 ACC cell lines, and the reactivation and more potent expression of RUNX3 was induced by 5-triazole-2-deoxycytidine. Our findings indicate that RUNX3 methylation may occur as a common event in the development of ACC and that methylation may be a major mechanism for inactivation of RUNX3 in ACC. AD - Department of Surgical Oncology, Zhejiang Province Cancer Hospital, Hangzhou 310022, P.R. China null Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Hangzhou 310022, P.R. China AU - Ge,Ming-Hua AU - Chen,Chao AU - Xu,Jia-Jie AU - Ling,Zhi-Qiang DA - 2011/08/01 DO - 10.3892/or.2011.1282 EP - 357 IS - 2 JO - Oncol Rep KW - human salivary gland adenoid cystic carcinoma runt-related transcription factor-3 methylation real-time methylation-specific PCR Western blotting disease-free survival PY - 2011 SN - 1021-335X 1791-2431 SP - 349 ST - Unfavorable clinical implications for hypermethylation of RUNX3 in patients with salivary gland adenoid cystic carcinoma T2 - Oncology Reports TI - Unfavorable clinical implications for hypermethylation of RUNX3 in patients with salivary gland adenoid cystic carcinoma UR - https://doi.org/10.3892/or.2011.1282 VL - 26 ER -