TY - JOUR AB - Using apoptosis-inducing factor protein as bait in a yeast hybrid assay to screen protein libraries, we identified three proteins that interacted with apoptosis-inducing factor: human homolog of yeast Rad23 protein A (hHR23A), microsomal glutathione S-transferase and caspase-14 (casp-14). In this study, we investigated the expression and function of casp-14 in lung adenocarcinomas (LADC). Our results showed that monoclonal antibodies were specific to casp-14, and that casp-14 was highly expressed in LADC. Casp-14 overexpression correlated with tumor stage, cell differentiation and lymphovascular involvement, suggesting that casp-14 was associated with tumor cell growth and metastatic potential. In vitro, casp-14 interacted with apoptosis-inducing factor, and silencing of casp-14 expression reduced cisplatin resistance. Our data suggest that casp-14 is an anti-apoptotic protein targeting apoptosis-inducing factor and increases cisplatin resistance in LADC cells. AD - Graduate Institute of Clinical Medicine, National Taiwan University School of Medicine, Taipei, Taiwan, R.O.C. null Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan, R.O.C Graduate Institute of Clinical Medicine, National Taiwan University School of Medicine, Taipei, Taiwan, R.O.C AU - Fang,Hsin-Yuan AU - Chen,Chih-Yi AU - Hung,Min-Fa AU - Hsiao,Yi-Ting AU - Chiang,Ting-Chieh AU - Lin,Tze-Yi AU - Chang,Hui-Wen AU - Chow,Kuan-Chih AU - Ko,Wen-Je DA - 2011/08/01 DO - 10.3892/or.2011.1292 EP - 369 IS - 2 JO - Oncol Rep KW - caspase-14 apoptosis-inducing factor cisplatin resistance lung adenocarcinoma anti-apoptosis PY - 2011 SN - 1021-335X 1791-2431 SP - 359 ST - Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas T2 - Oncology Reports TI - Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas UR - https://doi.org/10.3892/or.2011.1292 VL - 26 ER -