TY - JOUR AB - Evodiamine (Evo) is an indole quinazoline alkaloid isolated from the fruit of Evodia rutaecarpa Bentham. Previous studies have shown that Evo exhibits anti-proliferative anti-tumor activities in several cancer types, but its target(s) and underlying mechanism(s) of action remain unclear. In the present study, we sought to establish a cell synchronization model in order to examine the anti-proliferative and apoptotic mechanisms of Evo in the human gastric cancer cell line SGC-7901. In addition, we transfected these cells with full-length or non-degradable (ND) cyclinB1 to evaluate the relationship between the induction of apoptosis and activation of mitotic arrest and mitotic slippage by Evo. Our results demonstrated that Evo markedly inhibited cell growth and was cytotoxic to SGC-7901 cells. Furthermore, transient Evo treatment (<16 h) caused reversible mitotic arrest, but sustained mitotic arrest was required to initiate apoptosis. The time required to reverse the apoptotic effects of Evo was between 16 and 20 h. We also demonstrated that promotion of mitotic slippage by a CDK1 inhibitor enhanced apoptosis. Furthermore, we evaluated the effect of delaying mitotic slippage by overexpressing ND cyclinB1, which delayed apoptosis. In conclusion, these results indicate that Evo-induced apoptosis is associated with mitotic arrest and subsequent mitotic slippage, which may underlie the actions of Evo in the treatment and prevention of cancer. AD - Department of Pathophysiology, Medical College of Jinan University, Guangzhou 510632, P.R. China null College of Fundamental Medical Science, Guangzhou University of Chinese Medicine, no. 12 Jichang Road, Sanyuanli, Guangzhou 510405, P.R. China AU - Zhu,Li-Hong AU - Bi,Wei AU - Liu,Xiao-Dong AU - Li,Jie-Fen AU - Wu,Ying-Ya AU - Du,Biao-Yan AU - Tan,Yu-Hui DA - 2011/12/01 DO - 10.3892/or.2011.1444 EP - 1455 IS - 6 JO - Oncol Rep KW - evodiamine apoptosis mitotic arrest mitotic slippage cyclinB1 PY - 2011 SN - 1021-335X 1791-2431 SP - 1447 ST - Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage T2 - Oncology Reports TI - Induction of apoptosis by evodiamine involves both activation of mitotic arrest and mitotic slippage UR - https://doi.org/10.3892/or.2011.1444 VL - 26 ER -