TY - JOUR AB - Dihydroartiminisin (DHA), the active component of a Chinese herb (Artemisia annua), has been utilised as an anti-malarial drug since ancient China. DHA has also been shown to inhibit proliferation of cancer in vitro. However, the capacity of DHA to inhibit the development of ovarian cancer is still unclear. The adhesion, invasion, and migration of human ovarian cancer cell line (HO8910PM) was determined following DHA treatment in vitro, using Matrigel coated plate, transwell membrane chamber, and wound healing models, respectively. A mouse ovarian cancer model was established by orthotopic inoculation of HO8910PM cell line in nude mice. The growth and metastasis in vivo was determined 8 weeks post-implantation in response to DHA treatment. The expression of phosphorylated focal adhesion kinase (pFAK) and matrix metalloproteinases (MMP-2 and MMP-9) was evaluated using Western blotting. The expression of Von Willebrand factor (vWF) and infiltration of macrophages were determined, using immunohistochemistry. DHA inhibits ovarian cancer cell proliferation, adhesion, migration and invasion in vitro in a dose-dependent manner, consistent with decreased expression of pFAK and MMP-2, but not MMP-9. DHA inhibited metastasis significantly in vivo, associated with reduced vWF expression and macrophage infiltration. In conclusion, DHA inhibits the development of ovarian cancer, in part via down-regulating pFAK, MMP-2, vWF and macrophage infiltration. AD - Department of Obstetrics and Gynecology, Ren-Ji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, P.R. China null Discipline of Pathology, School of Medical Science and Bosch Institute, University of Sydney, NSW 2006 Australia Department of Obstetrics and Gynaecology, Ren-Ji Hospital, Shanghai Jiaotong University School of Medicine, 1630 Dong Fang Road, Shanghai 200127, P.R. China AU - Wu,Buchu AU - Hu,Ke AU - Li,Shu AU - Zhu,Jing AU - Gu,Liying AU - Shen,Haoran AU - Hambly,Brett ,D. AU - Bao,Shisan AU - Di,Wen DA - 2012/01/01 DO - 10.3892/or.2011.1505 EP - 108 IS - 1 JO - Oncol Rep KW - dihydroartiminisin ovarian cancer metastasis PY - 2012 SN - 1021-335X 1791-2431 SP - 101 ST - Dihydroartiminisin inhibits the growth and metastasis of epithelial ovarian cancer T2 - Oncology Reports TI - Dihydroartiminisin inhibits the growth and metastasis of epithelial ovarian cancer UR - https://doi.org/10.3892/or.2011.1505 VL - 27 ER -