TY - JOUR AB - The base excision repair gene MUTYH encodes glycosylase which removes adenine residues mispaired with 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHG). Biallelic germline mutations of the MUTYH gene are known to cause multiple colorectal adenomas including polyposis and cancer, mostly due to G:C➝T:A transversions in proto-oncogenes or tumor suppressor genes. The risk of colorectal cancer (CRC) in monoallelic mutation carriers of MUTYH is estimated to be higher in comparison with non-carriers. To investigate the possible role in sporadic CRC, we examined alterations of the MUTYH gene including somatic mutations and allelic loss in 101 cases of sporadic CRC, together with the KRAS mutation in some cases. MUTYH mutations in cancer DNA were detected in 3 cases, while mutations were also found in DNA samples from normal tissues, indicating that all were germline mutations. Allelic loss at the MUTYH locus was found in 10 of 51 (20.0%) CRC cases and KRAS mutations were found in 33 of the 101 (32.7%) samples. There was no significant difference in the rate of G:C➝T:A transversion in KRAS between cases with allelic loss (1 of 10, 10.0%) and without allelic loss (9 of 41, 22.0%). Investigation of quantitative allelic imbalance at SNP rs3219489 of MUTYH showed that CRC cases with C allele dominance (minor type corresponding to His) were more frequently detected with G:C➝T:A transversions than in those with G allele dominance (major type corresponding to Gln). In conclusion, somatic alterations of MUTYH in sporadic CRC were rare, similar to other DNA repair genes. However, it is possible that unknown mutations of regions not analyzed in this study and epigenetic changes of the promoter region of MUTYH may contribute to the disease. AD - Division of Lower Gastroenterology, Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan Department of Life Science, Faculty of Science and Engineering, Kinki University, Higashiosaka 577-8502, Japan AU - Kuno,Takashi AU - Matsubara,Nagahide AU - Tsuda,Satoshi AU - Kobayashi,Masayoshi AU - Hamanaka,Mie AU - Yamagishi,Daisuke AU - Tsukamoto,Kiyoshi AU - Yamano,Tomoki AU - Noda,Masafumi AU - Ikeuchi,Hiroki AU - Kim,Sanghyuk AU - Tamura,Kazuo AU - Tomita,Naohiro DA - 2012/08/01 DO - 10.3892/or.2012.1836 EP - 480 IS - 2 JO - Oncol Rep KW - sporadic colorectal cancer MUTYH gene base excision repair gene loss of heterozygosity PY - 2012 SN - 1021-335X 1791-2431 SP - 473 ST - Alterations of the base excision repair gene MUTYH in sporadic colorectal cancer T2 - Oncology Reports TI - Alterations of the base excision repair gene MUTYH in sporadic colorectal cancer UR - https://doi.org/10.3892/or.2012.1836 VL - 28 ER -