TY - JOUR AB - Nuclear factor (NF)-κB is the key transcription factor involved in the inflammatory responses, and its activation aggravates tumors. Peptidoglycan (PGN), a main cell wall component of Gram-positive bacteria, stimulates Toll-like receptor 2 (TLR-2) and activates a number of inflammatory pathways, including NF-κB. Cannabinoids have been reported to exert anti-inflammatory and antitumor effects. The mechanisms underlying these actions, however, are largely unknown. The purpose of this study was to investigate whether cannabinoids can suppress the PGN-induced activation of NF-κB and cell growth via cannabinoid receptors in U87MG human malignant glioma cells. PGN treatment induced the phosphorylation of NF-κB and cell proliferation in a concentration-dependent manner. The main endocannabinoid, 2-arachidonoylglycerol, prevented the PGN-induced phosphorylation of NF-κB, which was reversed by the CB1 cannabinoid receptor antagonist, AM281. The synthetic cannabinoid, WIN55,212-2, abolished the PGN-activated cell growth, and this effect was reversed by AM281. The preferential expression of CB1 rather than CB2 receptors in these cells was confirmed by reverse transcription-mediated polymerase chain reaction experiments and the observation that the WIN55,212-2-induced morphological changes were completely reversed by AM281 but not by the CB2 antagonist, AM630. Our finding that cannabinoids suppress the NF-κB inflammatory pathway and cell growth via CB1 receptors in glioma cells provides evidence for the therapeutic potential of targeting cannabinoid receptors for the treatment of inflammation-dependent tumor progression. AD - Department of Impairment Study, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan Department of Physiology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan Department of Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan AU - Echigo,Ryosuke AU - Sugimoto,Naotoshi AU - Yachie,Akihiro AU - Ohno-Shosaku,Takako DA - 2012/10/01 DO - 10.3892/or.2012.1937 EP - 1180 IS - 4 JO - Oncol Rep KW - cannabinoid nuclear factor-κB peptidoglycan cannab­inoid receptor 1 cell growth PY - 2012 SN - 1021-335X 1791-2431 SP - 1176 ST - Cannabinoids inhibit peptidoglycan-induced phosphorylation of NF-κB and cell growth in U87MG human malignant glioma cells T2 - Oncology Reports TI - Cannabinoids inhibit peptidoglycan-induced phosphorylation of NF-κB and cell growth in U87MG human malignant glioma cells UR - https://doi.org/10.3892/or.2012.1937 VL - 28 ER -