TY - JOUR AB - Chemokine receptors CCR6 and CCR7 have been reported to play important roles in T cell migration and organ-specific metastasis of various tumors. In the present study, we evaluated the expression and clinical significance of CCR6, CCR7, their ligands and CD4+CD25+Foxp3+ regulatory T cells in laryngeal squamous cell carcinoma (LSCC) and metastatic lymph nodes (LNs). The expression of CCR6, CCR7 and their ligands mRNA (CCL20, CCL19/CCL21) as well as the CCR6 and CCR7 proteins were detected by real-time RT-PCR and immunohistochemistry (IHC), respectively. Flow cytometry was used to investigate the percentage of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in peripheral blood mononuclear cells (PBMCs). Furthermore, a number of cytokines, including interleukin (IL)-2, IL-4, IL-10, IL-12p70, interferon (IFN)-γ and transforming growth factor (TGF)-β1 were detected by ELISA. The results showed that CCR6 and CCR7 were expressed in tumors in situ, metastatic LNs and CD4+CD25+Foxp3+ Tregs. It was hypothesized that the expression profile of CCR6, CCR7 and the proliferation of CD4+CD25+Foxp3+ Tregs affected the process of LN metastasis in LSCC patients. Therefore, the increased percentage of the Foxp3+ Tregs and the upregulation of Foxp3 expression on CCR6+ Tregs in LSCC patients may have accounted for the downregulation of antitumor immunity in these patients, which could be valuable for assessment of prognosis in LSCC treatment. AD - Department of Otolaryngology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, P.R. China Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China Department of Pathology, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, P.R. China AU - Chen,Bin AU - Zhang,Duo AU - Zhou,Jian AU - Li,Qing AU - Zhou,Lin AU - Li,Shi-Min AU - Zhu,Li AU - Chou,Kuang-Yen AU - Zhou,Liang AU - Tao,Lei AU - Lu,Li-Ming DA - 2013/09/01 DO - 10.3892/or.2013.2603 EP - 1390 IS - 3 JO - Oncol Rep KW - CCR6 CCR7 forkhead box P3 regulatory T cell laryngeal squamous cell carcinoma PY - 2013 SN - 1021-335X 1791-2431 SP - 1380 ST - High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma T2 - Oncology Reports TI - High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma UR - https://doi.org/10.3892/or.2013.2603 VL - 30 ER -