TY - JOUR AB - Gastric cancer is a common malignancy with a poor prognosis. β-elemene is a broad-spectrum anticancer drug extracted from the traditional Chinese medicinal herb Curcuma wenyujin. In the present study, we investigated the anticancer effects of β-elemene in gastric cancer cells and the potential proteins involved. Human SGC7901 and MKN45 gastric cancer cells were treated with different concentrations of β-elemene. Cell viability, clonogenic survival and apoptotic cell death were assessed. β-elemene inhibited viability and decreased clonogenic survival of gastric cancer cells in a dose-dependent manner. Apoptosis induction contributed to the anticancer effects. We then employed a proteomic method, isobaric tags for relative and absolute quantitation (iTRAQ), to detect the proteins altered by β-elemene. In total, 147 upregulated proteins and 86 downregulated proteins were identified in response to β-elemene treatment in SGC7901 gastric cancer cells. Among them, expression of p21-activated protein kinase‑interacting protein 1 (PAK1IP1), Bcl-2-associated transcription factor 1 (BTF) and topoisomerase 2-α (TOPIIα) were validated by western blot analyses and the trends were consistent with iTRAQ results. Top pathways involved in β-elemene treatment in SGC7901 gastric cancer cells included ribosome signaling, peroxisome proliferator-activated receptors (PPARs) signaling pathway, regulation of actin cytoskeleton, phagosome, biosynthesis and metabolism of some amino acids. Collectively, our results suggest a promising therapeutic role of β-elemene in gastric cancer. The differentially expressed proteins provide further insight into the potential mechanisms involved in gastric cancer treatment using β-elemene. AD - Department of General Surgery, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China AU - Liu,Jun-Song AU - He,Shi-Cai AU - Zhang,Zheng-Liang AU - Chen,Rui AU - Fan,Lin AU - Qiu,Guang‑Lin AU - Chang,Shuai AU - Li,Liang AU - Che,Xiang-Ming DA - 2014/12/01 DO - 10.3892/or.2014.3490 EP - 2647 IS - 6 JO - Oncol Rep KW - β-elemene gastric cancer proliferation apoptosis proteomics PY - 2014 SN - 1021-335X 1791-2431 SP - 2635 ST - Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study T2 - Oncology Reports TI - Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study UR - https://doi.org/10.3892/or.2014.3490 VL - 32 ER -