TY - JOUR AB - β-elemene, the active component of elemene (1-methyl-1-vinyl-2,4-diisopropenyl-cyclohexane), is a naturally occurring compound isolated from the traditional Chinese medicinal herb Curcuma wenyujin. Studies have confirmed that β-elemene enhances the radiosensitivity of lung cancer cell lines such as A549, by multiple pathways; however, their underlying mechanisms and pathways are yet to be elucidated. In the present study, two-dimensional differential in-gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry were used to profile the different proteins in A549 cell xenograft models of both treatment groups. The protein/mRNA expression was assessed by reverse transcription-polymerase chain reaction and western blotting techniques in tumor samples from all treatment groups. As a critical player in redox regulation of cancer cells, inhibition of peroxiredoxin-1 (Prx-1) may be an effective option for enhancing the tumor response to radiation. We further verified Prx-1 expression at the transcription and translation levels. β-elemene at a dose of 45 mg/kg had little effect on the Prx-1 protein expression, which was correlated with a moderate antitumor effect. However, a 45 mg/kg dose of β-elemene significantly inhibited the Prx-1 mRNA expression, thereby suggesting a possible influence on the transcriptional process, and radiation significantly increased the Prx-1 mRNA/protein expression compared to the control group (p<0.01). Notably, Prx-1 mRNA/protein expression was significantly lower in the β-elemene/radiation co-treatment group compared to the baseline levels in the control group (p<0.01). These results suggest that radiation-induced Prx-1 expression is directly or indirectly suppressed by β-elemene, thus suggesting a new pathway by which to reverse radioresistance. AD - Department of Radiation Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China Radiation Oncology Center, People's Liberation Army No. 323 Hospital, Xi'an, Shanxi 710000, P.R. China Department of Neurosurgery, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116001, P.R. China Department of Radiation Oncology, Ohio State University, Columbus, OH 43210-1219, USA AU - Li,Guoquan AU - Xie,Bingbing AU - Li,Xiaolong AU - Chen,Yinghai AU - Xu,Yinghui AU - Xu-Welliver,Meng AU - Zou,Lijuan DA - 2015/03/01 DO - 10.3892/or.2015.3732 EP - 1433 IS - 3 JO - Oncol Rep KW - lung cancer radiosensitivity β-elemene peroxiredoxin-1 PY - 2015 SN - 1021-335X 1791-2431 SP - 1427 ST - Downregulation of peroxiredoxin-1 by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenografts T2 - Oncology Reports TI - Downregulation of peroxiredoxin-1 by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenografts UR - https://doi.org/10.3892/or.2015.3732 VL - 33 ER -