TY - JOUR AB - RhoA regulates cell proliferation, migration, angiogenesis and gene expression. Altered RhoA activity contributes to cancer progression. The present study investigated the effects of RhoA knockdown on the regulation of ovarian cancer biological behavior in vitro and in nude mice. The expression of RhoA was knocked down using a lentivirus carrying RhoA short hairpin RNA (shRNA) in ovarian cancer cells and was confirmed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. The altered ovarian cancer biological behaviors were assayed by cell viability, terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL), migration, invasion, and nude mice tumorigenicity assays, while the altered gene expression was detected by RT-qPCR and western blot analysis. The results showed that lentivirus‑carrying RhoA shRNA significantly suppressed RhoA expression in ovarian cancer cells, which suppressed tumor cell viability, migration, invasion and adhesion in vitro. RhoA silencing also inhibited the tumorigenicity of ovarian cancer cells in nude mice, which was characterized by the suppression of tumor xenograft formation and growth and induction of tumor cell apoptosis. The results of the present study demonstrated that knockdown of RhoA expression had a significant antitumor effect on ovarian cancer cells in vitro and in nude mice, suggesting that RhoA may be a target for the development of a novel therapeutic strategy in the control of ovarian cancer. AD - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China Department of Obstetrics and Gynecology, Guangzhou First People's Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China Experimental Medical Research Center, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China AU - Wang,Xiaoxia AU - Jiang,Wenyan AU - Kang,Jiali AU - Liu,Qicai AU - Nie,Miaoling DA - 2015/08/01 DO - 10.3892/or.2015.4009 EP - 899 IS - 2 JO - Oncol Rep KW - ovarian cancer RhoA gene knockdown cell viability migration and invasion apoptosis tumor cell xenograft model PY - 2015 SN - 1021-335X 1791-2431 SP - 891 ST - Knockdown of RhoA expression alters ovarian cancer biological behavior in vitro and in nude mice T2 - Oncology Reports TI - Knockdown of RhoA expression alters ovarian cancer biological behavior in vitro and in nude mice UR - https://doi.org/10.3892/or.2015.4009 VL - 34 ER -