TY - JOUR AB - In the present study, the in vitro cytotoxicity of daidzein was evaluated in human BEL-7402, A549, HeLa, HepG-2 and MG-63 cancer cell lines. BEL-7402 cells were sensitive to daidzein treatment, with an IC50 value of 59.7±8.1 µM. Daidzein showed no cytotoxic activity toward A549, HeLa, HepG-2 and MG-63 cells. Daidzein increased the levels of reactive oxygen species (ROS) and induced a decrease in mitochondrial membrane potential. Morphological and comet assays showed that daidzein effectively induced apoptosis in BEL-7402 cells. Additionally, daidzein caused cell cycle arrest at the G2/M phase in the BEL-7402 cell line. Daidzein downregulated the expression of Bcl-2, Bcl-x and Baid proteins and upregulated the levels of Bim protein in the BEL-7402 cells. The results demonstrated that daidzein induced BEL-7402 cell apoptosis through an ROS-mediated mitochondrial dysfunction pathway. AD - School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China AU - Han,Bing-Jie AU - Li,Wei AU - Jiang,Guang-Bin AU - Lai,Shang-Hai AU - Zhang,Cheng AU - Zeng,Chuan-Chuan AU - Liu,Yun-Jun DA - 2015/09/01 DO - 10.3892/or.2015.4133 EP - 1120 IS - 3 JO - Oncol Rep KW - daidzein cytotoxicity in vitro reactive oxygen species mitochondrial membrane potential western blot analysis PY - 2015 SN - 1021-335X 1791-2431 SP - 1115 ST - Effects of daidzein in regards to cytotoxicity in vitro, apoptosis, reactive oxygen species level, cell cycle arrest and the expression of caspase and Bcl-2 family proteins T2 - Oncology Reports TI - Effects of daidzein in regards to cytotoxicity in vitro, apoptosis, reactive oxygen species level, cell cycle arrest and the expression of caspase and Bcl-2 family proteins UR - https://doi.org/10.3892/or.2015.4133 VL - 34 ER -