TY - JOUR AB - Studies have indicated that trichosanthin (TCS), a bioactive protein extracted and purified from the tuberous root of Trichosanthes kirilowii (a well‑known traditional Chinese medicinal plant), produces antitumor effects on various types of cancer cells. However, the effects of TCS on glioma cells are poorly understood. The objective of this study was to investigate the antitumor effects of TCS on the U87 and U251 cell lines. The in vitro effects of TCS on these two cell lines were determined using a Cell Counting Kit‑8 (CCK‑8) assay, Annexin V‑FITC staining, DAPI staining, Transwell assays, terminal deoxynucleotidyl transferase‑mediated dUTP nick end‑labeling (TUNEL) assays, 5,5',6,6'‑tetrachloro‑1,1',3,3'‑tetraethyl‑imidacarbocyanine iodide (JC‑1) staining and western blotting, which was utilized to assess the expression of leucine‑rich repeat‑containing G protein‑coupled receptor 5 (LGR5) and key proteins in the Wnt/β‑catenin signaling pathway. Our data indicated that TCS inhibited the proliferation of glioma cells in a dose‑ and time‑dependent manner and played a role in inhibiting glioma cell invasion and migration. Additional investigation revealed that the expression levels of LGR5 and of key proteins in the Wnt/β‑catenin signaling pathway were markedly decreased after TCS treatment. The results suggest that TCS may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/β‑catenin signaling pathway. In the future, in vivo experiments should be conducted to examine the potential use of this compound as a novel therapeutic agent for gliomas. AD - Department of Neurosurgery, Peking University People's Hospital, Beijing 100044, P.R. China AU - Miao,Junjie AU - Jiang,Yilin AU - Wang,Dongliang AU - Zhou,Jingru AU - Fan,Cungang AU - Jiao,Feng AU - Liu,Bo AU - Zhang,Jun AU - Wang,Yangshuo AU - Zhang,Qingjun DA - 2015/12/01 DO - 10.3892/or.2015.4290 EP - 2852 IS - 6 JO - Oncol Rep KW - trichosanthin glioma apoptosis LGR5 Wnt/β‑catenin signaling PY - 2015 SN - 1021-335X 1791-2431 SP - 2845 ST - Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/β-catenin signaling pathway T2 - Oncology Reports TI - Trichosanthin suppresses the proliferation of glioma cells by inhibiting LGR5 expression and the Wnt/β-catenin signaling pathway UR - https://doi.org/10.3892/or.2015.4290 VL - 34 ER -