TY - JOUR AB - Hydrogen sulfide (H2S) participates in diverse physiological and pathophysiologic processes of cancer both in vitro and in vivo. We have previously reported the proliferation/anti-apoptosis/angiogenesis/migration effects of exogenous H2S on liver cancer and glioma via amplifying the activation of NF-κB and p38 MAPK/ERK1/2-COX-2 pathway. However, the effects of H2S on EC109 esophageal cells remain unclear. The present study demonstrated the effects of exogenous H2S on cancer cell growth via activating HSP90 pathways in EC109 esophageal cells. EC109 esophageal cells were treated with 400 µmol/l NaHS (a donor of H2S) for 24 h. The expression levels of HSP90, bcl-2, caspase-3, bax and MMP-2 were detected by western blot assay. Cell viability was detected by Cell Counting Kit-8 (CCK-8). The migration rate was analyzed using a Transwell migration assay and ImageJ software. NaHS promoted cell proliferation, as evidenced by an increase in cell viability. In addition, NaHS treatment reduced apoptosis, as indicated by the increased bcl-2 expression and decreased cleaved caspase-3 and bax expression. Importantly, exposure of NaHS increased the expression of MMP-2, the migration rate and expression of VEGF. Notably, co-treatment of EC109 cells with NaHS and GA (an inhibitor of HSP90 pathway) largely suppressed the aforementioned NaHS-induced effects. The findings of the present study provided novel evidence that HSP90 pathway was involved in NaHS-induced cancer cell proliferation, anti-apoptosis, angiopoiesis and migration in EC109 esophageal cells. AD - Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangdong, Guangzhou 510080, P.R. China Department of Oncology, The Affiliated Hospital, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China Department of Cardiovasology and Cardiac Care Unit (CCU), Huangpu Division of The First Affiliated Hospital, Sun Yat-sen University, Guangdong, Guangzhou 510700, P.R. China Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, P.R. China AU - Lei,Yiyan AU - Zhen,Yulan AU - Zhang,Wei AU - Sun,Xiuting AU - Lin,Xiaoxiong AU - Feng,Jianqiang AU - Luo,Honghe AU - Chen,Zhenguang AU - Su,Chunhua AU - Zeng,Bo AU - Chen,Jingfu DA - 2016/06/01 DO - 10.3892/or.2016.4734 EP - 3720 IS - 6 JO - Oncol Rep KW - hydrogen sulfide EC109 cells HSP90 pathway growth PY - 2016 SN - 1021-335X 1791-2431 SP - 3714 ST - Exogenous hydrogen sulfide exerts proliferation, anti-apoptosis, angiopoiesis and migration effects via activating HSP90 pathway in EC109 cells T2 - Oncology Reports TI - Exogenous hydrogen sulfide exerts proliferation, anti-apoptosis, angiopoiesis and migration effects via activating HSP90 pathway in EC109 cells UR - https://doi.org/10.3892/or.2016.4734 VL - 35 ER -