TY - JOUR AB - Arctigenin is a bioactive lignan isolated from the seeds of Arctium lappa L. which has been widely used as a diuretic and a diaphoretic in Traditional Chinese Medicine. In the present study, the authors investigated the effects of arctigenin on tumor migration and invasion in aggressive human breast cancer cells. The MTT assay results showed that arctigenin did not show a significant cytotoxic effect on the cell viability of MDA-MB-231 cells. However, wound healing migration and Boyden chamber invasion assays demonstrated that arctigenin significantly inhibited in vitro migration and invasion of the MDA-MB-231 cells. Furthermore, gelatin zymography results showed that arctigenin reduced the activity of MMP-2 and MMP-9. Western blot analysis results demonstrated that the expression of MMP-2, MMP-9 and heparanase proteins was significantly downregulated following the treatment of arctigenin. Finally, the antiangiogenic activity of arctigenin was also examined by the chick embryo chorioallantoic membrane (CAM) assay. Arctigenin treatment significantly inhibited angiogenesis in the CAM. In conclusion, the results revealed that arctigenin significantly inhibited the migration and invasion of MDA-MB-231 cells by downregulating MMP-2, MMP-9 and heparanase expression. However, further studies are still necessary to investigate the exact mechanisms involved and to explore signal transduction pathways to better understand the biological mechanisms. AD - College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Binjiang, Hangzhou, Zhejiang 310053, P.R. China AU - Lou,Chenghua AU - Zhu,Zhihui AU - Zhao,Yaping AU - Zhu,Rui AU - Zhao,Huajun DA - 2017/01/01 DO - 10.3892/or.2016.5269 EP - 184 IS - 1 JO - Oncol Rep KW - arctigenin breast cancer metastasis matrix metallo­proteinases heparanase PY - 2017 SN - 1021-335X 1791-2431 SP - 179 ST - Arctigenin, a lignan from Arctium lappa L., inhibits metastasis of human breast cancer cells through the downregulation of MMP-2/-9 and heparanase in MDA-MB-231 cells T2 - Oncology Reports TI - Arctigenin, a lignan from Arctium lappa L., inhibits metastasis of human breast cancer cells through the downregulation of MMP-2/-9 and heparanase in MDA-MB-231 cells UR - https://doi.org/10.3892/or.2016.5269 VL - 37 ER -