TY - JOUR AB - MicroRNA-361-5p (miR-361-5p) has been reported to be dysregulated in various human cancer types. However, the function of miR-361-5p in glioma remains unknown. In the present study, we aimed to investigate the biological functions of miR-361-5p in regulating glioma progression and the underlying molecular mechanism. We found that miR-361-5p was significantly decreased in glioma tissues and cell lines as detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. Functional analysis revealed that miR-361-5p overexpression significantly inhibited glioma cell migration, invasion and epithelial-mesenchymal transition (EMT) whereas suppression of miR-361-5p showed opposite effects. Bioinformatic analysis showed that Twist1, a critical EMT inducer, was a predicted target of miR-361-5p which was validated by dual-luciferase reporter assay, RT-qPCR and western blot analysis. Further analysis indicated that miR-361-5p regulates the Twist1/Bmi-1 signaling axis. Rescue experiments showed that restoration of Twist1 expression significantly reversed the suppressive effect of miR-361-5p on cell migration, invasion and EMT. Taken together, the present study demonstrated an important role of miR-361-5p in glioma - which regulated the EMT of glioma cells by targeting and regulating Twist1. These findings provide novel insight into understanding the role and mechanism of miR-361-5p in regulating the biolo­gical behavior of glioma cells and suggest that miR-361-5p is a novel potential therapeutic target for glioma. AD - Department of Neurosurgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China Department of Clinical Laboratory, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China AU - Zhang,Xi AU - Wei,Chunyan AU - Li,Jin AU - Liu,Jiali AU - Qu,Jianqiang DA - 2017/03/01 DO - 10.3892/or.2017.5406 EP - 1856 IS - 3 JO - Oncol Rep KW - glioma miR-361-5p Twist1 epithelial-mesenchymal transition PY - 2017 SN - 1021-335X 1791-2431 SP - 1849 ST - MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1 T2 - Oncology Reports TI - MicroRNA-361-5p inhibits epithelial-to-mesenchymal transition of glioma cells through targeting Twist1 UR - https://doi.org/10.3892/or.2017.5406 VL - 37 ER -