TY - JOUR AB - A growing body of evidence suggests that microRNA-365 (miR-365) played crucial role in the initiation and development of many types of cancers. However, the biological role of miR-365 in human glioma remains unclear. Herein, the aims of this study were to investigate the role and underlying mechanisms of miR-365 in glioma by a series of in vitro and in vivo experiments. We found that miR-365 was strongly downregulated in malignant glioma tissues and cell lines. Restoration of the expression of miR-365 in glioma cells significantly inhibited cell proliferation, migration and invasion in vitro and tumor growth in vivo. Notably, phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was proved to be a direct target of miR-365 in glioma cells, and its mRNA expression was inversely correlated with miR-365 expression in clinical glioma tissues. PIK3R3 overexpression in miR-365 expressing cells could rescue proliferation, migration and invasion inhibition of miR-365. In addition, miR-365 was able to inhibit the phosphorylation of AKT and mTOR in vitro and in vivo, which are key participants in the AKT/mTOR pathway. These results suggest that miR-365 functioned as a tumor suppressor in glioma by targeting PIK3R3, suggesting that miR-365 has potential as therapeutic targets for glioma. AD - Department of Radiotherapy, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China Department of Nuclear Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China Department of Gastroenterology, The People's Republic Hospital of Jilin Province, Changchun, Jilin 130021, P.R. China Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China AU - Zhu,Yonggang AU - Zhao,Hongguang AU - Rao,Min AU - Xu,Songbai DA - 2017/04/01 DO - 10.3892/or.2017.5458 EP - 2192 IS - 4 JO - Oncol Rep KW - glioma miR-365 proliferation invasion PIK3R3 PY - 2017 SN - 1021-335X 1791-2431 SP - 2185 ST - MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3 T2 - Oncology Reports TI - MicroRNA-365 inhibits proliferation, migration and invasion of glioma by targeting PIK3R3 UR - https://doi.org/10.3892/or.2017.5458 VL - 37 ER -