TY - JOUR AB - Colon cancer is common worldwide and accounts for the significant cancer related morbidity and mortality in patients. Although extensive advancement has been made in colon cancer treatment and diagnosis in the last decades, there is still a giant gap between the clinical expectation. It has been reported that resveratrol (Res) may be a potential candidate for cancer treatment. However, the specific mechanism underlying this activity remains unclear. In this study, we investigated the anticancer activity of Res in human colon cancer cells, and unveiled the possible mechanism for this effect. With cell viability, flow cytometry, PCR and western blot analysis, we demonstrated the efficacious anticancer activity of Res in HCT116 cells. Mechanically, we found that Res greatly upregulates BMP7 in HCT116 cells. Exogenous BMP7 enhances the anticancer effect of Res in HCT116 cells, which was almost reversed by the BMP7 specific antibody. Res does not activate the BMPs/Smads signaling, but decreases the phosphorylation of Akt1/2/3 substantially in HCT116 cells. Exogenous BMP7 enhances the inhibitory effect of Res on the phosphorylation of Akt1/2/3, while BMP7 immunodepletion reverses this effect notably. Res markedly decreases the phosphorylation of PTEN, which can be enhanced by exogenous BMP7 but partly reversed by the BMP7 antibody. Our findings suggested that Res may be a promising candidate for colon cancer treatment, and the anticancer activity may be mediated by inactivating PI3K/Akt signaling through upregulating BMP7 to decrease, at least, the phosphorylation of PTEN. AD - Department of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing 400016, P.R. China Chongqing Key Laboratory for Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, P.R. China AU - Zeng,Yu-Hua AU - Zhou,Lin-Yun AU - Chen,Qian-Zhao AU - Li,Yang AU - Shao,Ying AU - Ren,Wen-Yan AU - Liao,Yun-Peng AU - Wang,Han AU - Zhu,Jia-Hui AU - Huang,Ming AU - He,Fang AU - Wang,Jin AU - Wu,Ke AU - He,Bai-Cheng DA - 2017/07/01 DO - 10.3892/or.2017.5662 EP - 464 IS - 1 JO - Oncol Rep KW - resveratrol antiproliferation colon cancer BMP7 PI3K/Akt PTEN PY - 2017 SN - 1021-335X 1791-2431 SP - 456 ST - Resveratrol inactivates PI3K/Akt signaling through upregulating BMP7 in human colon cancer cells T2 - Oncology Reports TI - Resveratrol inactivates PI3K/Akt signaling through upregulating BMP7 in human colon cancer cells UR - https://doi.org/10.3892/or.2017.5662 VL - 38 ER -