TY - JOUR AB - Anti-angiogenesis is one of the most general clinical obstacles in cancer chemotherapy. Kaempferol is a flavonoid phytochemical found in many fruits and vegetables. Our previous study revealed that kaempferol triggered apoptosis in human umbilical vein endothelial cells (HUVECs) by ROS‑mediated p53/ATM/death receptor signaling. However, the anti‑angiogenic potential of kaempferol remains unclear and its underlying mechanism warranted further exploration in VEGF‑stimulated HUVECs. In the present study, kaempferol significantly reduced VEGF‑stimulated HUVEC viability. Kaempferol treatment also inhibited cell migration, invasion, and tube formation in VEGF‑stimulated HUVECs. VEGF receptor‑2 (VEGFR‑2), and its downstream signaling cascades (such as AKT, mTOR and MEK1/2‑ERK1/2) were reduced as determined by western blotting and kinase activity assay in VEGF‑stimulated HUVECs after treatment with kaempferol. The present study revealed that kaempferol may possess angiogenic inhibition through regulation of VEGF/VEGFR‑2 and its downstream signaling cascades (PI3K/AKT, MEK and ERK) in VEGF-stimulated endothelial cells. AD - Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, R.O.C. Medical Education Center, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, R.O.C. Department of Dermatology, E‑Da Hospital and I‑Shou University, Kaohsiung 82445, Taiwan, R.O.C. Department of Pharmacy, Buddhist Tzu Chi General Hospital, Hualien 97002, Taiwan, R.O.C. Department of Nursing, Tajen University, Pingtung 90741, Taiwan, R.O.C. Department of Pharmacy, Tajen University, Pingtung 90741, Taiwan, R.O.C. Huamn Genetics Center, Department of Medical Research, China Medical University Hospital, Taichung 40447, Taiwan, R.O.C. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan, R.O.C. AU - Chin,Hsien‑Kuo AU - Horng,Chi‑Ting AU - Liu,Yi‑Shan AU - Lu,Chi‑Cheng AU - Su,Chen‑Ying AU - Chen,Pei‑Syuan AU - Chiu,Hong‑Yi AU - Tsai,Fuu‑Jen AU - Shieh,Po‑Chuen AU - Yang,Jai‑Sing DA - 2018/05/01 DO - 10.3892/or.2018.6312 EP - 2357 IS - 5 JO - Oncol Rep KW - angiogenesis kaempferol VEGF receptor‑2 AKT MEK/ERK human umbilical vein endothelial cells (HUVECs) PY - 2018 SN - 1021-335X 1791-2431 SP - 2351 ST - Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells T2 - Oncology Reports TI - Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells UR - https://doi.org/10.3892/or.2018.6312 VL - 39 ER -